塔里木大学学报2025，Vol.37Issue(6)：24-34,11.DOI:10.3969/j.issn.1009-0568.2025.06.003
甜瓜内生菌和根际土微生物的多样性及抑菌活性筛选
Diversity and antimicrobial activity of endophytic bacteria and rhizosphere soil microorganisms from Cucumis melo L
摘要
Abstract
The aim of this study is to investigate the diversity of endophytic and rhizosphere soil microorganisms of Cucumis melo L.in Southern Xinjiang,and screen for strains with antibacterial activity against the pathogens of muskmelon fusarium wilt and bacterial fruit blotch of melon.Microorganisms were isolated from the roots,stems,leaves,and rhizosphere soil of Cucumis melo L.using the dilution plate method.The diversity of endophytic and rhizosphere soil microorganisms in Cucumis melo L.was identified through bacterial 16S rRNA and fungal ITS gene sequencing.The antibacterial activity of ethyl acetate crude extract of the fermentation broth of the isolated strains was determined by punching method,against the pathogens of muskmelon fusarium wilt and bacterial fruit blotch of melon.21 bacteria of 12 genera and 15 species and 1 fungus were isolated from the roots of Cucumis melo L.;19 bacteria of 4 genera and 4 species and 2 fungi of 2 genera and 2 species were isolated from the stems;1 bacterium was isolated from the leaves;26 bacteria of 16 genera and 22 species and 9 fungi of 4 genera and 8 species were isolated from the rhizosphere soil.Microbacterium is the most dominant genus.There were difference in species richness among different tissues of Cucumis melo L.,in the rhizosphere soil>root>stem>leaf.Antibacterial activity screening showed that 11 strains had antibacterial activity against the pathogen of bacterial fruit blotch of melon,and 6 strains(LG-11,T-1,T-2,T-6,T-8 and T-26)had antibacterial zones with diameters over 30 mm.This study revealed the diversity of endophytic and rhizosphere soil microorganisms of Cucumis melo L.in Southern Xinjiang,which provides resources for the development of safe and environmentally friendly microbial agents for controlling Cucumis melo L.related diseases.关键词
甜瓜/内生菌/根际土微生物/多样性/抑菌活性Key words
Cucumis melo L./endophytic bacteria/rhizosphere soil microorganisms/diversity/antimicrobial activity分类
生物科学引用本文复制引用
SUN Beibei,ZHOU Yu,WU Can,WANG Lijun..甜瓜内生菌和根际土微生物的多样性及抑菌活性筛选[J].塔里木大学学报,2025,37(6):24-34,11.基金项目
新疆生产建设兵团指导性科技计划项目(2022ZD115) （2022ZD115）
塔里木大学校长基金一般项目(TDZKYB202203) （TDZKYB202203）
