土壤学报2025，Vol.62Issue(6)：1734-1744,11.DOI:10.11766/trxb202412270511
农药百菌清对土壤N2O排放的影响及机制
Effects of the Addition of Pesticide Chlorothalonil on Soil N2O Emissions and Related Mechanisms
摘要
Abstract
[Objective]The heavy use of pesticides such as chlorothalonil(CTN)in agricultural production inevitably has an impact on the soil's ecological environment.Farmland soils are considered an important emission source of greenhouse gasses nitrous oxide(N2O).However,there are few studies on how CTN affects agricultural soil N2O emission and its associated microbial mechanism.[Method]In this study,the response characteristics of N2O emission in three kinds of farmland soils(S1-vegetable field soil,S2-rice field soil,and S3-wheat field soil)to different concentrations of CTN were studied through indoor culture experiments.The concentration gradients of chlorothalonil were set as 0(CK),5(T5),10(T10)and 25 mg·kg-1(T25).The abundance of microbial functional genes was analyzed simultaneously through real-time quantitative PCR.[Result]The results showed that(1)Compared with CK,the treatments of T5,T10,and T25 in the acidic vegetable field soil of S1 led to an increase of 1 868%,1 264%,and 232%in N2O emissions,respectively.In the S2 neutral paddy soil,T5,T10,and T25 respectively led to an increase of 4%,138%,and 334%in N2O emissions,while for the alkaline wheat field soil S3,T5,T10,and T25 led to an increase of 230%,119%,and 23%in N2O emissions respectively.(2)The addition of CTN changed soil physicochemical and microbial characteristics,and significantly increased soil dissolved organic carbon(DOC)content,ammonia-oxidizing archaea(AOA),ammonia-oxidizing bacteria(AOB),nitrite-reducing bacteria(nirK,nirS),N2O reducing bacteria(nosZ),and total bacteria(16S rRNA)genes abundance.Correlation analysis showed that the increase in N2O emissions was mainly related to the increase in soil carbon and nitrogen substrate contents and the abundance of microbial functional genes,and was mainly attributed to the increase in the gene abundance of total bacteria,ammonia-oxidizing bacteria,and nir-type denitrifying bacteria,as well as the increase in soil DOC content.[Conclusion]CTN can increase soil DOC content and microbial gene abundance,and eventually lead to an increase in soil N2O emission.Also,the effect of CTN on soil N2O emission depends on soil properties and application concentration.The results of this study provide a scientific basis for further understanding of soil N2O emission driven by CTN application and its microbiological mechanism and also have important significance for understanding the potential ecological and environmental risks of agricultural CTN application.关键词
农田土壤/农药百菌清/氧化亚氮/功能基因/可溶性有机碳(DOC)Key words
Cropland soil/Pesticide chlorothalonil/Nitrous oxide/Functional genes/Dissolved organic carbon(DOC)分类
农业科技引用本文复制引用
ZHANG Jingdi,ZOU Jianwen,LIU Shuwei..农药百菌清对土壤N2O排放的影响及机制[J].土壤学报,2025,62(6):1734-1744,11.基金项目
江苏省碳达峰碳中和科技创新专项资金项目(BE2023398)和中央高校基本科研业务费专项资金项目(KJJQ2025016)资助 Supported by the Technology Innovation Special Fund for Carbon Dioxide Emission Peaking and Carbon Neutrality of Jiangsu Province,China(No.BE2023398)and the Fundamental Research Funds for the Central Universities,China(No.KJJQ2025016) （BE2023398）
