畜牧兽医学报2025，Vol.56Issue(12)：5943-5951,9.DOI:10.11843/j.issn.0366-6964.2025.12.001
单细胞转录组测序技术在家禽中的研究进展与发展趋势
Research Advances and Future Perspectives of Single-Cell RNA Sequencing Technology in Poultry
摘要
Abstract
Single-cell RNA sequencing(scRNA-seq)is revolutionizing poultry biology research by resolving cellular heterogeneity and dynamic gene expression at single-cell resolution.This review systematically summarizes the advances in scRNA-seq applications for poultry:the tech-nology enables cell-by-cell analysis,offering novel strategies to enhance egg production efficien-cy,improve meat quality,and boost disease resistance.However,current research faces bottle-necks including incomplete cellular atlas coverage,scarce functional validation systems,and high agricultural conversion costs.Future efforts should prioritize developing low-cost sequencing,in-tegrating multi-omics data(e.g.,scATAC-seq and metabolomics),and implementing targeted validation via CRISPR/Cas9-mediated avian models.These approaches will drive intelligent de-sign of precision breeding and reproductive regulation.This review provides theoretical founda-tions and technical prospects for deciphering cell fate determination mechanisms in poultry and their practical applications.关键词
单细胞转录组测序/禽类卵泡发育/产蛋性状/家禽育种Key words
single-cell RNA sequencing/avian follicular development/egg-laying traits/poultry breeding分类
农业科技引用本文复制引用
DONG Tianshu,LUAN Yan,KE Wentao,LIANG Hao,LI Shaoming,LI Peng,WANG Jiaxiang,WU Yan,PI Jinsong,XIE Yang..单细胞转录组测序技术在家禽中的研究进展与发展趋势[J].畜牧兽医学报,2025,56(12):5943-5951,9.基金项目
国家重点研发计划资助(2022YFD1600100) （2022YFD1600100）
国家现代农业产业技术体系资助(CARS-40 （）
CARS-41 （）
CARS-42) （）
湖北省支持种业高质量发展资金项目(HBZY2023B007) （HBZY2023B007）
国家自然科学基金项目(32202665) （32202665）
湖北省重大攻关项目(2023BAA029) （2023BAA029）
