现代中西医结合杂志2025，Vol.34Issue(21)：2941-2947,7.DOI:10.3969/j.issn.1008-8849.2025.21.003
电针对肥胖大鼠骨骼肌组织ADRB2、NR4A3表达的影响
Effects of electroacupuncture on ADRB2 and NR4A3 expressions in skeletal muscle of obese rats
摘要
Abstract
Objective It is to observe the effect of obesity on skeletal muscle glucose uptake and the effects of electroa-cupuncture(EA)on the expressions of β2 adrenergic receptor(ADRB2),nuclear receptor subfamily 4 group A member 3(NR4A3)and glucose transporter type 4(GLUT4)in skeletal muscle of high-fat diet(HFD)-induced obese rats.Meth-ods Thirty male Wistar rats were taken,in which 10 rats were randomly selected as normal group and fed with standard di-et;the 20 remaining rats were fed with high-fat diet to establish models of obesity.The successfully modeled rats were divid-ed into normal model group and EA group,with 10 rats in each group.The rats in the EA group were treated with Han's electroacupuncture device at Zhongwan(CV12)-Guanyuan(CV4),Zusanli(ST36)-Fenglong(ST40)(forming a dual-circuit stimulation system),2 Hz continuous wave with an current intensity of 1 mA,20 min per time,3 times weekly,continuously treated for 8 weeks.The body weight,fasting plasma glucose(FPG),2-hour postprandial glucose(2hPG)of the rats were measured before and after treatment,the levels of glycated hemoglobin(HbA1c),serum total cholesterol(TC),triglycerides(TG)and free fatty acids(FFA)were measured by fully automated biochemical analyzer after 8 weeks of treatment,the protein expressions of ADRB2 and GLUT4 in skeletal muscle tissue were detected by Western blot,the mRNA expressions of ADRB2,NR4A3 and GLUT4 in skeletal muscle tissue were detected by fluorescent quantitative PCR,the positive expression of ADRB2 in skeletal muscle tissue was detected by immunohistochemistry.Results Before interven-tion and after 8 weeks of intervention,the body weight,FPG,and 2hPG of rats in the model group and EA group were sig-nificantly higher than those in the normal group at the same time point(all P<0.05);after 8 weeks of intervention,the body weight,FPG,and 2hPG of rats in the EA group were significantly lower than those in the model group(all P<0.05).Compared with the normal group,the serum levels of HbA1c,TG,and FFA were significantly increased,while the relative protein expressions of ADRB2 and GLUT4,relative mRNA expressions of ADRB2,NR4A3 and GLUT4 and the ADRB2-positive area fraction in skeletal muscle tissue of rats were all significantly reduced in the model group(all P<0.05).Compared with the model group,the serum levels of HbA1c,TG,and FFA were significantly decreased,while the relative protein expressions of ADRB2 and GLUT4,relative mRNA expressions of ADRB2,NR4A3 and GLUT4 and the ADRB2-positive area fraction in skeletal muscle tissue of rats were all significantly increased in the EA group(all P<0.05).Conclusion Electroacupuncture can promote glucose uptake in skeletal muscle of high-fat diet-fed obese rats,the mechanism may be related to upregulating expressions of ADRB2 and NR4A3 in skeletal muscle,thereby promoting expres-sion of GLUT4.关键词
肥胖/电针/β2肾上腺素受体/核受体亚家族4成员3/葡萄糖转运蛋白4Key words
obesity/glucose uptake/electroacupuncture/β2 adrenergic receptor/nuclear receptor subfamily 4 group A member 3/glucose transporter type 4分类
医药卫生引用本文复制引用
XUE Tingzheng,YANG Shurui,WANG Yayuan,HUANG Liuyang,TIAN Haoran,HAN Yushen,KANG Chenyang,WANG Jiaqi,LIANG Fengxia..电针对肥胖大鼠骨骼肌组织ADRB2、NR4A3表达的影响[J].现代中西医结合杂志,2025,34(21):2941-2947,7.基金项目
国家自然科学基金资助项目(82274634) （82274634）
