现代中西医结合杂志2025，Vol.34Issue(21)：2955-2961,2967,8.DOI:10.3969/j.issn.1008-8849.2025.21.005
基于CD86+/CD163+巨噬细胞比值探讨健脾祛邪汤联合吉西他滨对原位种植人胰腺癌裸鼠肿瘤生长的影响
Decoction for fortifying spleen and eliminating pathogenic factors combined with gemcitabine inhibits tumor growth in nude mice with orthotopic transplantation human pancreatic cancer through increasing CD86+/CD163+macrophage
摘要
Abstract
Objective It is to observe the effect and mechanism of decoction for fortifying spleen and eliminating patho-genic factors combined with gemcitabine(GEM)on the tumor growth and ratio of CD86+/CD163+macrophage in nude mice with orthotopic transplantation human pancreatic cancer(PC),and to explore its mechanism of action.Methods Or-thotopic transplantation human pancreatic cancer(PC)models were established by subcutaneous implantation of PANC-1 human PC cell lines into nude mice followed by pancreatic transplantation.One week later,40 nude mice with implanted tumors were randomly divided into 4 groups:model group,GEM group,low-dose and high-dose groups of decoction for for-tifying spleen and eliminating pathogenic factors+GEM,with 10 mice in each group.The low-dose and high-dose groups of decoction for fortifying spleen and eliminating pathogenic factors+GEM were given decoction for fortifying spleen and elimi-nating pathogenic factors containing 1.32 g/mL and 2.64 g/mL crude drug by gavage,respectively,the model group and GEM group were given an equivalent volume of normal saline by gavage,all once daily.All the groups except for the model group were given GEM 25 mg/kg by intraperitoneal injection,twice weekly.All the groups were administered continuously for 4 weeks.In one week after intervention,the body weight and tumor mass were measured,and the tumor suppression rate was calculated,the serum levels of carcinoembryonic antigen(CEA)and carbohydrate antigen 19-9(CA19-9)were measured by ELISA,the morphology of implanted tumor cells was observed by HE staining,the infiltration of CD68+,CD86+,and CD163+macrophage in implanted tumor tissues was detected by immunohistochemistry.Results The tumor suppression rates of the GEM group,low-dose and high-dose groups of decoction for fortifying spleen and eliminating patho-genic factors+GEM were 41.29%,56.13%and 70.32%,respectively.Compared with the model group,the tumor weight,serum levels of CEA and Ca19-9,average optical density of CD68+,CD163+in the implanted tumor tissue and the ratio of CD163+/CD68+macrophage in the GEM group were significantly decreased(all P<0.05),while the average optical density of CD86+,the ratios of CD86+/CD68+and CD86+/CD163+M were significantly increased(all P<0.05);HE staining showed that there were focal damage and necrosis of tumor cells in the implanted tumor tissue of the GEM group.Compared with the GEM group,the tumor weight,serum levels of CEA and Ca19-9,average optical densities of CD68+,CD163+in the low-dose and high-dose groups of decoction for fortifying spleen and eliminating pathogenic fac-tors+GEM were significantly decreased(all P<0.05),while the average optical density of CD86+,the ratios of CD86+/CD68+and CD86+/CD163+macrophage were significantly increased(all P<0.05);HE staining showed that there were more damage and necrosis of tumor cells in the two combination groups.Conclusion Decoction for fortifying spleen and e-liminating pathogenic factors can enhance the inhibitory effect of GEM on tumor growth in the nude mice with orthotopic transplantation human pancreatic cancer,the mechanism might be related to the regulation of macrophage species and in-crease of ratio of CD86+/CD163+macrophage.关键词
胰腺癌/健脾祛邪汤/吉西他滨/巨噬细胞/CD86/CD163Key words
pancreatic cancer/decoction for fortifying spleen and eliminating pathogenic factors/gemcitabine/macro-phages/CD86/CD163分类
医药卫生引用本文复制引用
CHEN Junmei,ZHAO Hui,LI Yanjun,ZHANG Ying,SHENG Dongyun,TENG Yao..基于CD86+/CD163+巨噬细胞比值探讨健脾祛邪汤联合吉西他滨对原位种植人胰腺癌裸鼠肿瘤生长的影响[J].现代中西医结合杂志,2025,34(21):2955-2961,2967,8.基金项目
武汉市卫生健康委员会面上项目(WZ22C03) （WZ22C03）
