右江医学2025，Vol.53Issue(12)：1075-1080,6.DOI:10.3969/j.issn.1003-1383.2025.12.003
肝纤维化小鼠原代肝细胞的分离培养
Isolation and cultivation of primary hepatocytes from mice with liver fibrosis
摘要
Abstract
Objective To establish a reliable method for isolating and purifying primary liver cells from mice with high vitality and purity of liver fibrosis.Methods The classical two-step collagenase perfusion method was improved by using in-ferior vena cava perfusion,intermittent clamping of the portal vein perfusion,and 50％Percoll density-gradient centrifugation for purification of hepatocyte suspension.The purified hepatocytes were cultured in vitro,and immunofluorescent staining of Albumin and HNF1α was performed.Results The survival rate of primary liver cells isolated and purified from liver fibrosis mice could stably reach(92.93±1.93)％,with an average total number of live cells of(1-2)×107 per adult mouse.Purified cells adhered to the substrate and began to spread within 2 h of in vitro culture.Conclusion A stable tech-nique for isolating and culturing primary liver cells from mice with high purity and vitality in liver fibrosis is successfully established.关键词
肝纤维化/小鼠肝细胞/高纯度肝细胞/体外培养技术Key words
liver fibrosis/mouse hepatocytes/high purity hepatocytes/in vitro culture technique分类
医药卫生引用本文复制引用
WANG Hong,LIAO Zhengquan,CHEN Wenping,ZHANG Sheng..肝纤维化小鼠原代肝细胞的分离培养[J].右江医学,2025,53(12):1075-1080,6.基金项目
广东省医学科研基金立项项目(B2024037) （B2024037）
广东省医学科学技术研究基金项目(B2021163) （B2021163）
