西南林业大学学报2026，Vol.46Issue(1)：65-73,9.DOI:10.11929/j.swfu.202411056
山新杨GATA基因家族成员鉴定及盐胁迫下基因表达分析
Identification and Expression Analysis of the GATA Gene Family in Populus davidiana×P.alba var.pyramidalis Under Salt Stress
摘要
Abstract
To investigate the role of GATA transcription factors in salt stress response,we characterized the PdaGATAs gene family in Populus davidiana×P.alba var.pyramidalis using transcriptome database analysis and bioinformatics tools.Bioinformatics analysis was used to examine the physicochemical properties,phylogeny,conserved motifs of PdaGATAs,and the expression patterns was explored by RT-qPCR in was different tissues under salt stress.The results show that 24 GATA transcription factor genes were identified in Populus davidiana×P.alba var.pyramidalis.Phylogenetic analysis and multiple sequence alignment revealed that PdaGATAs were classified into 4 subfamilies,all containing a conserved zinc finger domain with high structural homology within subfamilies.PdaGATAs have close relationships with GATA members from Populus trichocarpa and Populus eu-phratica,with promoters harboring hormone and stress-responsive elements.RT-qPCR results demonstrated tis-sue-specific expression patterns:PdaGATA18 showed highest expression in stems,PdaGATA1,2,and 20 were significantly expressed in roots,and most PdaGATAs exhibited high expression levels in leaves.Under salt stress,expression profiles of PdaGATAs changed significantly,with notable upregulation of PdaGATA4,7,13,14,and 15,suggesting their critical regulatory roles in salt stress adaptation in Populus davidiana×P.alba var.pyramidalis.关键词
山新杨/GATA基因/家族基因/盐胁迫/表达模式Key words
Populus davidiana×P.alba var.pyramidalis/GATA gene/gene family/salt stress/expression pattern分类
农业科技引用本文复制引用
Deng Weiqi,Chen Yang,Han Youzhen,Wang Fusen,Xing Zhenghua,Zhao Xin,Wang Chao..山新杨GATA基因家族成员鉴定及盐胁迫下基因表达分析[J].西南林业大学学报,2026,46(1):65-73,9.基金项目
科技创新2030-重大项目(2023ZD0405603)资助 （2023ZD0405603）
国家级大学生创新创业训练项目(202310225106)资助. （202310225106）
