西南林业大学学报2026，Vol.46Issue(1)：74-80,7.DOI:10.11929/j.swfu.202503017
旱柳叶绿体基因组的密码子偏好性特征分析
Characterization of Codon Usage Bias in the Chloroplast Genome of Salix matsudana
摘要
Abstract
Salix matsudanais is a plant of both ecological and economic value.To uncover the codon usage bias(CUB)and its driving factors in the chloroplast genome of S.matsudana,CodonW and CUSP software were used to analyze the codon usage patterns of 51 filtered protein-coding sequences.The results showed that the GC content of the GC1,GC2,GC3 and the GCall content of the codons in the chloroplast genome of S.matsudana were 45.85%,37.77%,29.41%,and 37.67%,respectively.The effective number of codons(ENC)values ranged from 33.59 to 57.39,with an average of 45.49,and 33 genes had ENC values exceeding 45,indicating weak codon us-age bias in most protein-coding sequences.Additionally,32 codons had relative synonymous codon usage(RSCU)values≥1,and these codons were more likely to end with A or U.Further neutrality plot,ENC-plot,and PR2-plot analyses suggested that mutation and natural selection commonly influenced codon usage bias,with natural selec-tion being the predominant factor.Through the analysis,13 optimal codons were identified in the chloroplast gen-ome of S.matsudana,providing theoretical support for its genetic improvement and gene engineering applications.关键词
旱柳/密码子偏好性/最优密码子/叶绿体基因组Key words
Salix matsudana/codon usage bias/optimal codon/chloroplast genome分类
农业科技引用本文复制引用
Zou Dajie,Guo Borui,Liu Jiaqian,Lu Xingxing,Sun Yuying,Wang Jiuli..旱柳叶绿体基因组的密码子偏好性特征分析[J].西南林业大学学报,2026,46(1):74-80,7.基金项目
青海省科技重大专项项目(2023-SF-A5)资助. （2023-SF-A5）
