眼科学报2025，Vol.40Issue(11)：964-970,7.DOI:10.12419/251204
病原宏基因组测序技术在眼部感染性疾病中的应用与实验室经验分享
Application of pathogen metagenomic sequencing in ocular infectious diseases:laboratory experience and insights
摘要
Abstract
Based on laboratory experience,this article reviews the applications and challenges of metagenomic next-generation sequencing(mNGS)in ocular infectious diseases.While mNGS serves as a valuable tool for diagnosing difficult infections due to its unbiased detection capabilities,it is not yet suitable as a first-line routine test given its complex workflow and interpretation requirements.Ocular specimens,characterized by trace nucleic acid amounts,high diversity,and complex backgrounds,present unique technical bottlenecks.To address these,this article proposes key strategies,including retaining human nucleic acids during wet-lab processing,constructing a localized background microbial library,and utilizing host data for concurrent tumor screening.The study emphasizes that strengthening multidisciplinary teams(MDT)between clinical and laboratory departments is essential to promote the standardization and precision of mNGS applications in ophthalmology.关键词
病原宏基因组测序/眼部感染/眼部标本Key words
pathogen metagenomic sequencing(mNGS)/ocular infection/ocular specimens分类
医药卫生引用本文复制引用
ZHANG Pei,FENG Lina,WANG Yanling,LI Yingyu..病原宏基因组测序技术在眼部感染性疾病中的应用与实验室经验分享[J].眼科学报,2025,40(11):964-970,7.基金项目
中国初级卫生保健基金会研究专项(MTP2022B017).This work is supported by China Primary Health Care Foundation,Research Project(MTP2022B017). （MTP2022B017）
