医学新知2025，Vol.35Issue(12)：1398-1405,8.DOI:10.12173/j.issn.1004-5511.202507023
CD226在子痫前期胎盘中的表达及其对滋养细胞功能的研究
Expression of CD226 in preeclamptic placentas and its role in trophoblast function
摘要
Abstract
Objective To analyze the expression characteristics of CD226 in placental tissues from preeclampsia(PE)patients and its biological function in trophoblast cells.Methods CD226 mRNA transcript levels and protein expression in placental tissues from the PE patients and women with normal pregnancies were detected by qRT-PCR and immunohistochemical staining.In the trophoblast cell lines HTR8/SVneo and JAR,CD226 gene was knocked down or overexpressed,respectively,to evaluate the impact of its expression changes on cell proliferation,apoptosis,migration,and invasion.Western blot analysis was used to detect the effects of CD226 on the protein expression of TNF-α and NF-κB p65.Results Compared with the normal pregnancy group,the expression level of CD226 was significantly up-regulated in the placental tissues of the PE group.Knockdown of CD226 enhanced the proliferation,migration,and invasion,and suppressed apoptosis in HTR8/Svneo cells.Conversely,the overexpression of CD226 significantly inhibited the proliferation,migration,and invasion of JAR cells,while promoting apoptosis in these cells.Furthermore,Western blot analysis revealed that CD226 overexpression promoted the protein expression of TNF-α and NF-κB p65 in trophoblast cells.Conclusion The expression level of CD226 in placental tissues of PE is higher than that in normal pregnancy group.CD226 exerts the inhibitory effects on the proliferation,migration,and invasion,and promotes apoptosis in trophoblast cells.关键词
子痫前期/CD226/胎盘组织/增殖/侵袭Key words
Preeclampsia/CD226/Placental tissue/Proliferation/Invasion分类
医药卫生引用本文复制引用
YU Yang,BAI Jinbo,FENG Dan,XU Xiaoyan,WANG Bo,LIU Zekun,ZHANG Ming..CD226在子痫前期胎盘中的表达及其对滋养细胞功能的研究[J].医学新知,2025,35(12):1398-1405,8.基金项目
国家自然科学基金青年科学基金项目(82203336) （82203336）
西北妇女儿童医院院级一般项目(2022YN06) （2022YN06）
