烟台大学学报（自然科学与工程版）2026，Vol.39Issue(1)：42-49,8.DOI:10.13951/j.cnki.37-1213/n.241101
乳铁蛋白修饰的烟酰胺单核苷酸脂质体的制备与评价
Preparation and Evaluation of Lactoferrin-Modified Niacinamide Mononucleotide Liposomes
摘要
Abstract
In order to improve the brain targeting of nicotinamide mononucleotide(NMN)and enhance its blood-brain barrier permeability,lactoferrin-modified nicotinamide mononucleotide liposomes(NMN-Lip-Lf)were con-structed and prepared using a multi-emulsion method combined with a functional modification strategy.First,the formulation and process parameters of the liposome were optimized using single factor experiment and Box-Behnken response surface method.The optional conditions were as follows:membrane lipid ratio of 1∶9.5,lipid concentra-tion of 4 mg/mL,and a molar ratio of DPE-PEG2000 was 3%.The ultrasonic emulsification conditions were opti-mized:an internal water phase/organic phase volume ratio 1∶4.3,an internal water phase/external water phase volume ratio 1∶9,ultrasonic power 200 W,two emulsification durations of 4.5 min and 1 min,respectively,and vacuum evaporation parameters(32℃for 10 min).Further,DSPE-PEG2000-COOH was partially replaced with DSPE-PEG2000 via an esterification reaction and coupled with lactoferrin to prepare functionalized liposomes.The results showed that the particle size of NMN-Lip-Lf was(147.40±5.49)nm,the PDI was 0.26±0.05,the Zeta potential was-(8.87±0.41)mV,and the encapsulation efficiency and drug loading were(25.11±1.35)%and(5.02±0.27)%,respectively.The colloidal dispersion exhibited good stability,and the in vitro release study showed a slow-release profile.The results of the in vitro blood-brain barrier model experiment showed that the blood-brain barrier permeability of NMN-LiP-LF was 6.2-fold and 4.7-fold of original drug(NMN)and unmodified liposome(NMN-Lip)respectively.These results provide a new strategy for the development and application of NMN targeted delivery systems in the central nervous system.关键词
烟酰胺单核苷酸/乳铁蛋白/脂质体/复乳法/Box-Behnken响应面法/血脑屏障Key words
nicotinamide mononucleotide/lactoferrin/liposome/double emulsion method/Box-Behnken response surface method/blood-brain barrier分类
医药卫生引用本文复制引用
ZHAO Fengdong,PING Songyuge,JIANG Yuanyuan,WU Kaihan,LIN Yuxian,FAN Yutong,XU Hui..乳铁蛋白修饰的烟酰胺单核苷酸脂质体的制备与评价[J].烟台大学学报（自然科学与工程版）,2026,39(1):42-49,8.基金项目
浙江省"尖兵领雁"研发攻关计划项目(2022C03145) （2022C03145）
烟台大学实验室开放基金项目(2024-2025学年). （2024-2025学年）
