山西医科大学学报2025，Vol.56Issue(11)：1210-1218,9.DOI:10.13753/j.issn.1007-6611.2025.11.002
小鼠心脏衰老的转录组重塑及组织学特征
Transcriptomic remodeling and histological characteristics of cardiac aging in mice
摘要
Abstract
Objective To investigate molecular features and histological changes associated with cardiac aging in mice,and screen key genes and signaling pathways related to aging for providing references for the study on the mechanism of cardiac aging and the screening of intervention targets.Methods Ten healthy male C57BL/6 mice were divided into young group(8 weeks old)and old group(20 months old),with 5 mice in each group.Illumina high-throughput transcriptome sequencing was employed to compare and analyze gene expression differences in myocardial tissue between the two groups.Combined with bioinformatics analysis methods such as DESeq2,differentially expressed genes were screened,and the pathway enrichment analysis was performed using the KEGG data-base.HE staining was used to observe changes in myocardial tissue structure,and immunofluorescence double labeling was employed to detect the protein expression and spatial co-localization of α-SMA with CDH4,SLC38A1,and SLC38A4.ImageJ and GraphPad Prism software were utilized to conduct statistical analysis of data.Results Transcriptome analysis revealed that,compared with young group,499 upregulated genes and 138 downregulated genes were detected in old group.The differentially expressed genes were significantly enriched in immune-and metabolism-related pathways,such as complement and coagulation cascades,and cytokine-cy-tokine receptor interactions.HE staining results showed a significant increase in the cross-sectional area of cardiomyocytes in old group compared to young group(P<0.05),and a increased trend of perimeter(P=0.084).Meanwhile,immunofluorescence results indicated a significant increase of α-SMA expression(P<0.01),and strengthened spatial co-localization with CDH4,SLC38A1,and SLC38A4 in the aged myocardial tissue.Conclusion Mouse cardiac aging involves molecular remodeling of multiple signaling pathways re-lated to immune response,metabolism,and cell adhesion,and is accompanied by fibroblast activation and changes in tissue structure.SLC38A1,CDH4,and SLC38A4 could serve as potential new targets for interventions against cardiac aging.关键词
心脏衰老/转录组测序/基因表达/免疫荧光/组织重塑/纤维化Key words
cardiac aging/transcriptome sequencing/gene expression/immunofluorescence/tissue remodeling/fibrosis分类
医药卫生引用本文复制引用
ZHANG Ling,LI Mi,MA Wenshuai,LI Wuping,MA Sicong,WANG Jin,MA Litian,ZHANG Zhewei..小鼠心脏衰老的转录组重塑及组织学特征[J].山西医科大学学报,2025,56(11):1210-1218,9.基金项目
陕西省自然科学基础研究计划项目(2021JQ-349) （2021JQ-349）
陕西省自然科学基础研究计划面上项目(2021JM-082) （2021JM-082）
