中草药2026，Vol.57Issue(1)：45-52,8.DOI:10.7501/j.issn.0253-2670.2026.01.006
钟花龙胆中2个新酚苷类成分
Two new phenolic glycosides from Gentiana nanobella
摘要
Abstract
Objective To study the chemical constituents fromdried whole plant of alpine herb Gentiana nanobella,and to conduct preliminary screening for antimalarial activity of the isolated constituents.Methods Ethyl acetate and n-butanol fractions of 75%ethanol extract of the whole plant of G.nanobella were isolated and purified by repeated column chromatography padded with silica gel,RP-18 and Sephadex LH-20,respectively.The structures of the isolated compounds were identified based on their physicochemical properties and spectral data.The in vitro inhibitory activity of the testing compound against human Plasmodium falciparum 3D7 strain was achieved with the SYBR Green I method.Results A total of 11 phenol compounds were obtained and identified as 2,3-dihydroxy benzoic acid methyl ester-3-O-β-L-rhamnosyl-(1″→2′)-β-D-glucopyranoside(1),2,3-dihydroxy benzoic acid-3-O-β-L-rhamnosyl-(1″→2′)-β-D-glucopyranoside(2),2,3-dihydroxy benzoic acid methyl ester-3-O-β-D-glucopyranosyl-(1″→6′)-β-D-glucopyranoside(3),benzyl β-D-xylopyranosyl-(1″→6′)-β-D-glucopyranoside(4),apigenin 5-O-β-D-glucopyranoside(5),salcolin B(6),apigenin-7-O-β-D-glucopyranoside(7),benzoic acid(8),p-hydroxybenzoic acid(9),3-hydroxy-2-methoxybenzoic acid(10),and isovanillic acid(11).Compound 6 exhibited an in vitro antimalarial activity against Plasmodium falciparum 3D7 strain with IC50 18.50 μmol/L and inhibition rate of 98.48%at a concentration of 50 μmol/L.Conclusion Compounds 1 and 2 are new compounds,named gentinanoside A and gentinanoside B.Compounds 1-11 were isolated from G.nanobella for the first time.G.nanobella is abundant in phenolic compounds,and some compounds had certain antimalarial activity.关键词
钟花龙胆/新酚苷类化合物/抗疟活性/钟花龙胆苷A/钟花龙胆苷B/芹菜素-5-O-β-D-吡喃葡萄糖苷Key words
Gentiana nanobella C.Marquand/new phenolic glycosides/antimalarial activity/gentinanoside A/gentinanoside B/apigenin 5-O-β-D-glucopyranoside分类
医药卫生引用本文复制引用
YANG Dawei,LING Haowei,WU Jiu,GOU Liang,XU Wei,JIANG Bei..钟花龙胆中2个新酚苷类成分[J].中草药,2026,57(1):45-52,8.基金项目
国家自然科学基金项目(81960637,82460689) （81960637,82460689）
云南省滇西抗病原植物资源筛选研究重点实验室建设项目及研究生开放课题项目(202305AG340015,APR2024Y17) （202305AG340015,APR2024Y17）
