中国癌症杂志2025，Vol.35Issue(12)：1119-1125,7.DOI:10.19401/j.cnki.1007-3639.2025.12.004
胃癌伴神经内分泌肿瘤与单纯型胃癌的免疫微环境特征比较研究
A study on the immunomicroenvironment characteristics of gastric cancer with neuroendocrine neoplasm and pure gastric cancer
ZHANG Yi 1SUN Haidong 2QU Xinglong 2ZHAO Bin3
作者信息
- 1. Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200137,China||Department of Surgical Oncology,Minhang Branch,Fudan University Shanghai Cancer Center,Shanghai 201100,China
- 2. Department of Surgical Oncology,Minhang Branch,Fudan University Shanghai Cancer Center,Shanghai 201100,China
- 3. Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200137,China
- 折叠
摘要
Abstract
Background and purpose:Gastric cancer with neuroendocrine neoplasm(GC-NEN)accounts for a relatively high proportion of gastric cancers.This article mainly studied the immune microenvironment of pure gastric carcinoma(PGC)and GC-NEN,and expounded the differences of immune microenvironment between them.Methods:Based on the samples of gastric cancer with neuroendocrine tumors collected from Fudan University Shanghai Cancer Center Minhang Branch between November 21,2021 and March 5,2024,along with stage-,age-and sex-matched PGC samples showing no statistically significant differences in these baseline variables,we performed seven-color multiplex fluorescent immunohistochemistry(CD8,PD-L1,FOXP3,CD163,CD34,CKpan and DAPI).The quantitative data or spatial distribution differences of relevant indexes in tumor-related regions(tumor region and stromal region)were analyzed by HALO software,and the differences in tumor immune microenvironment between the two were explained.Results:A total of 11 PGC patient samples and 15 GC-NEN patient samples were included in this study.The results of quantitative analysis showed that the proportion of CD8+and PD-L1+cells was higher in the stromal region of GC-NEN tissue samples than in PGC tissue samples,and the proportion of CD34+cells was lower in tumor region than in PGC tissue samples.There was a positive correlation between the infiltration of different immune cells in both PGC and GC-NEN tissues.The results of spatial analysis showed that the ES scores of PD-L1+CKpan-cells interacting with tumors in GC-NEN tissue samples were higher than those of PGC patients,the EP scores of PD-L1+cells were higher than those of PGC patients,and the EP scores of CD34+and CD163+PD-L1-cells were lower than those of PGC tissue samples.Conclusion:There are some significant differences in the tumor immune microenvironment between GC-NEN patients and PGC patients in the indicators we tested.The relationship between this difference and the prognosis of patients and immunotherapy needs to be further explored.关键词
胃癌/胃癌伴神经内分泌/肿瘤免疫微环境/免疫排斥Key words
Gastric cancer/Gastric cancer with neuroendocrine neoplasm/Tumor immune microenvironment/Immune exclusion分类
医药卫生引用本文复制引用
ZHANG Yi,SUN Haidong,QU Xinglong,ZHAO Bin..胃癌伴神经内分泌肿瘤与单纯型胃癌的免疫微环境特征比较研究[J].中国癌症杂志,2025,35(12):1119-1125,7.
