中国输血杂志2025，Vol.38Issue(12)：1662-1668,1694,8.DOI:10.13303/j.cjbt.issn.1004-549x.2025.12.002
西双版纳傣族自治州献血者登革病毒筛查情况分析
Analysis of Dengue virus nucleic acid testing screening among blood donors in Xishuangbanna Dai Autonomous Pre-fecture,China
摘要
Abstract
Objective To investigate the prevalence of Dengue virus(DENV)infection among voluntary blood donors in Xishuangbanna Dai Autonomous Prefecture,and to evaluate the necessity of implementing nucleic acid testing(NAT)for blood donors during the rainy season(May-October).Methods Prior to initiating donor screening,the Xishuangbanna Central Blood Center conducted in-house validation of reagent performance and participated in external quality assessment(EQA)organized by the National Center for Clinical Laboratories(NCCL).During the surveillance period(August-Octo-ber 2024),a total of 2 919 donor samples were screened using a 6-sample mini-pool NAT strategy.Daily internal quality controls were recorded.Samples that tested positive in pooled screening were deconvoluted and retested in duplicate;only those reactive in both replicate wells were sent to the NCCL for confirmatory testing.At NCCL,samples underwent re-testing using five domestic NAT reagents,as well as serological assays for NS1 antigen and DENV-specific IgG/IgM.Confirmed positive samples were further characterized by serotyping,envelope(E)gene sequencing,and phylogenetic analysis using the maximum likelihood method.Results The DENV NAT reagent demonstrated consistent detection of 40 copies/mL con-trols in individual donor(ID)-NAT test(mean CT:35.61±0.40).During the 63-day quality control monitoring,DENV detection remained stable(mean CT:22.53±0.72).The center achieved full marks in EQA assessments for 2023 and 2024.Three reactive pools were identified in initial screening,and subsequent individual testing confirmed three DENV RNA-positive donors(sample numbers:2401,2402,and 2403).The confirmatory test results from NCCL were:all five NAT platforms consistently detected DENV RNA in the three samples;for serological tests,2 samples(2402,2403)were posi-tive for NS1 antigen,while all three samples were negative for both IgG and IgM antibodies.DENV serotyping reagents iden-tified DENV-2 in all cases,which were further confirmed as DENV-2 Genotype Ⅱ-Cosmopolitan by E gene sequencing.Phylogenetic analysis indicated that samples 2401 and 2402 clustered with Southeast Asian strains(Thailand/MZ636802.1,Laos/PQ775621.1),while sample 2403 closely matched a previously reported local Yunnan strain(PV544686.1).Con-clusion DENV-2 infection was detected among blood donors in Xishuangbanna during the rainy season,indicating concur-rent risks of imported and local transmission.We recommend implementing pooled NAT screening for blood donors in high-risk areas during dengue epidemic seasons,along with strengthened laboratory quality control,to enhance blood safety.关键词
西双版纳傣族自治州/献血者/登革病毒/核酸检测Key words
Xishuangbanna Dai Autonomous Prefecture/blood donors/Dengue virus/nucleic acid testing(NAT)分类
医药卫生引用本文复制引用
LIU Xinru,DENG Mingwen,GAO Xiaoqian,WANG Lunan,LU Shaofang,YAN Ying,DONG Jing,WU Ji,MA Jie,CHANG Le,JI Huimin,SUN Huizhen..西双版纳傣族自治州献血者登革病毒筛查情况分析[J].中国输血杂志,2025,38(12):1662-1668,1694,8.基金项目
中国医学科学院医学与健康科技创新工程(CIFMS)(2021-I2M-1-060) （CIFMS）
