中国草地学报2025，Vol.47Issue(12)：57-67,11.DOI:10.16742/j.zgcdxb.20250117
生物炭与氮肥配施对混播草地土壤微生物群落的影响
Effects of Biochar and Nitrogen Fertilizer Application on Soil Microbial Communities in Mixed Grasslands
摘要
Abstract
Biochar has shown great potential for improving soil physicochemical properties and enhanc-ing crop productivity.However,the mechanisms by which the combined application of biochar and nitro-gen fertilizer influences forage yield,soil nutrient dynamics,and soil microbial communities in legume-grass mixed pastures remain unclear.In this study,a mixed pasture of alfalfa(Medicago sativa)and smooth bromegrass(Bromus inermis)was used to examine the effects of biochar and nitrogen fertilizer co-application on soil physicochemical properties,microbial biomass,diversity,and community structure,as well as their interrelationships.The results showed that the combined application of biochar and nitrogen fertilizer significantly enhanced soil nutrient availability.Compared with application of nitrogen fertilizer alone at 180 kg/hm2,the treatment of 8 t/hm2 biochar plus 180 kg/hm2 nitrogen fertilizer increased soil total nitrogen by 35.71%,ammonium nitrogen by 42.19%,and available potassium by 34.38%(P<0.05),while no significant difference was observed in total phosphorus content.Biochar addition markedly altered soil microbial community structure,increasing the number of unique bacterial ASVs(e.g.,10 372 in the 8 t/hm2 biochar plus 180 kg/hm2 nitrogen treatment)and elevating the relative abundance of Actino-bacteria,Chloroflexi,Gemmatimonadota,and Sphingomonas.Alpha diversity analysis indicated that nitro-gen fertilization alone reduced bacterial richness,whereas biochar addition had no significant effect on alpha diversity indices.Correlation analysis revealed that variations in the relative abundance of different soil bac-terial groups were primarily associated with soil total nitrogen,nitrate nitrogen,available potassium,and forage yield.In conclusion,the combined application of 8 t/hm2 biochar and 180 kg/hm2 nitrogen fertilizer significantly improves soil fertility,promotes nutrient cycling,and enhances forage productivity.These findings provide a theoretical basis for the use of biochar-nitrogen integration to support degraded grassland restoration and sustainable pasture management.关键词
生物炭/氮肥/豆禾混播/土壤微生物群落/土壤养分Key words
Biochar/Nitrogen fertilizer/Legume-grass mixed pastures/Soil microbial community/Soil nutrients分类
农业科技引用本文复制引用
ZHAO Chen,GAO Shikong,ZHAO Jie,HAO Xiaojun,GAO Junjun,HU Tianming,FU Juanjuan..生物炭与氮肥配施对混播草地土壤微生物群落的影响[J].中国草地学报,2025,47(12):57-67,11.基金项目
神木市"优质牧草生产与加工技术的研究与示范"项目 （）
