中华耳科学杂志2026，Vol.24Issue(1)：58-62,5.DOI:10.3969/j.issn.1672-2922.2026.01.011
饱和氢生理盐水预防小鼠噪声性听力损失的实验研究
Protective effects of hydrogen-saturated saline on noise-induced hearing loss in mice
摘要
Abstract
Objective The purpose of this study is to investigate potential roles of saturated hydrogen saline in prevention of noise-induced hearing loss(NIHL).Methods Twenty-four 8-week-old specific pathogen free(SPF)grade C57BL/6J male mice with normal auditory brainstem response(ABR)thresholds were randomly divided into normal control(wild type,WT),noise exposure(NG),and saturated hydrogen saline prophylaxis before noise exposure(HS)groups(n=8 in each group).The control group received intraperitoneal saline solution(1 mL/100 g/day)for three days.The NG group received the same saline treatment followed by noise exposure,whereas the HS group received an equivalent dose of saturated hydrogen saline before noise exposure.ARB audiometry was performed prior to and at 1,3 and 7 days after noise exposure.At the conclusion of the study,the middle cochlear turn basilar membrane was harvested and examined by scanning electron microscopy with immunofluorescence staining.Results Hearing loss was observed in both the NG and HS groups,with better ABR thresholds,less severe cochlear outer hair cells damage and less outer hair cells loss in the HS group than in the NG group(P<0.05).The degree of damage to the cilia morphology of cochlear outer hair cellsin the HS group was milder than that in the NG group.Cochlear basilar membrane ribbon synapses decreased in both HS and NG groups,and less in the NG group than in the HS group(P>0.05).Conclusions Prophylactic daily intraperitoneal injection of saturated hydrogen saline has a protective effect on hearing in mice against exposure to steady-state noise.This knowledge may have certain value in understanding molecular mechanisms of hydrogen protection.关键词
稳态噪声/噪声性听力损失/饱和氢生理盐水Key words
steady state noise/noise-induced hearing loss/saturated hydrogen saline引用本文复制引用
TANG Jinge,REN Wei,WEI Cong,QI Guowei,SHI Lei,JIANG Qingqing,YUAN Shuolong,ZHU Yuhua,YU Ning..饱和氢生理盐水预防小鼠噪声性听力损失的实验研究[J].中华耳科学杂志,2026,24(1):58-62,5.基金项目
国家重点研发项目(2020YFC2004001) （2020YFC2004001）
北京市自然科学基金项目(7222185) （7222185）
