中国中药杂志2025，Vol.50Issue(24)：6851-6860,10.DOI:10.19540/j.cnki.cjcmm.20250814.303
基于血清代谢组学研究煅牡蛎标准汤剂与配方颗粒治疗酒精性胃溃疡大鼠的等效性与作用机制
Equivalence and mechanism of standard decoction and formula granules of calcined oyster in treatment of rats with ethanol-induced gastric ulcers based on serum metabolomics
摘要
Abstract
An ethanol-induced gastric ulcer model was replicated to investigate the equivalence and metabolic regulatory mechanisms of formula granules and standard decoction of calcined oyster by using metabolomics.The ethanol-induced gastric ulcer model was established,and the dose-effect curve was fitted with the ulcer inhibition rate as the effect to compare the equal-effect dose(DEE)and equal-dose effect(EED)of standard decoction and formula granules of calcined oyster.The formula granule group and standard decoction group with the equivalent effect were selected for further pharmacodynamic index detection,and metabolomics analysis was performed on the serum to explore the metabolic regulation mechanism of formula granules and standard decoction of calcined oyster.According to the dose-effect curve equation,the DEE and EED of formula granules and standard decoction were calculated.Formula granules and standard decoction of calcined oyster could improve the body's oxidative stress and inflammatory factor level,increase the level of protective factors,and treat ethanol-induced gastric ulcers.Serum metabolomics analysis showed that formula granules and standard decoction of calcined oyster mainly treated ethanol-induced gastric ulcers through eight potential metabolic pathways,and the metabolic regulation of standard decoction and formula granules on gastric ulcer was different.Within the corresponding effective range,1 g·kg-1 standard decoction was pharmacologically equivalent to 1.07 g·kg-1 formula granules in treating ethanol-induced gastric ulcers.Formula granules and standard decoction of calcined oyster mainly treat ethanol-induced gastric ulcers by regulating metabolic disorders in pathways such as primary bile acid biosynthesis.关键词
代谢组学/煅牡蛎/等效性/胃溃疡Key words
metabolomics/calcined oyster/equivalence/gastric ulcer引用本文复制引用
LIU Yan-chang,ZENG Zhi-hao,XIAO Guan-lin,JIANG Jie-yi,LI Yang-xue,LI Su-mei,BI Xiao-li..基于血清代谢组学研究煅牡蛎标准汤剂与配方颗粒治疗酒精性胃溃疡大鼠的等效性与作用机制[J].中国中药杂志,2025,50(24):6851-6860,10.基金项目
广东省基础与应用基础研究基金项目(2022A1515220171) （2022A1515220171）
