仲恺农业工程学院学报2026，Vol.39Issue(1)：1-10,10.DOI:10.3969/j.issn.1674-5663.2026.01.001
花生咖啡酸3-O甲基转移酶基因AhCOMT的克隆与生物信息学分析
Cloning and bioinformatics analysis of AhCOMT gene of peanut caffeic acid 3-O methyltransferase
摘要
Abstract
The phenylpropane metabolic pathway plays an important role in the plant growth stages and in tackling abiotic stress.This study cloned a key caffeic acid 3-O-methyltransferase gene,AhCOMT,involved in lignin synthesis in the phenylpropanoid metabolism pathway.The CDS gene is 1 119 bp in length and encodes 403 amino acids.The protein has a molecular weight of 44 371.69 Da and an isoelectric point pI of 6.89.There is no transmembrane structure,and subcellular localization predicts that the protein is located in chloroplasts and mitochondria,making it a hydrophilic protein.There are 17 known cis-acting elements related to plant growth,development,and stress resistance in the promoter region.Analysis of the expression pattern of the AhCOMT gene showed that it was expressed in different tissues and organs with varying levels,especially in peanut seeds,where the expression level was highest.Compared with the control group,root aluminum treatment could induce AhCOMT gene expression.The interaction between AhCOMT and the upstream transcription factor protein AhMYB86,predicted using AlphaFold3,was analyzed.This study lays the foundation for the function of the AhCOMT gene and provides a basis for further research on potential resistance gene resources for cultivating high-quality peanut varieties in the future.关键词
花生/铝胁迫/苯丙烷代谢途径/咖啡酸 3-O甲基转移酶Key words
peanut/aluminum stress/phenylpropane metabolic pathway/caffeic acid 3-O-methyl-transferase分类
农业科技引用本文复制引用
BAO Gegen,DING Chao,HUANG Yafei,LI Lin,YU Jiayan,WAN Xiaorong,YANG Pisheng,ZHENG Yixiong..花生咖啡酸3-O甲基转移酶基因AhCOMT的克隆与生物信息学分析[J].仲恺农业工程学院学报,2026,39(1):1-10,10.基金项目
广东省科技厅农村科技计划区域创新能力与支撑保障体系建设专项资金(2023B0202010025)、"十四五"广东省农业科技创新十大主攻方向"揭榜挂帅"项目(2024SDZG05)、广东省自然科学基金(2024A1515012997)、广东省科学技术厅农村科技特派员项目(KTP20240077)、广东省基础与应用基础研究基金广州市联合基金(粤穗联合基金)(2022A1515111059)、广州市科技局基础研究项目(SL2023A04J01391)、仲恺农业工程学院研究生科技创新基金(KJCX2024037、KJCX2024020)和仲恺农业工程学院大学生创新训练计划项目(X202411347275). （2023B0202010025）
