仲恺农业工程学院学报2026，Vol.39Issue(1)：11-15,5.DOI:10.3969/j.issn.1674-5663.2026.01.002
香蕉线条病毒GF SYBR Green Ⅰ实时荧光定量PCR检测方法的建立
Establishment of SYBR Green Ⅰ real time fluorescence quantitative PCR method for detecting banana streak virus GF
摘要
Abstract
Banana streak virus GF(BSGFV)is a major banana streak virus(BSV)that infects bananas.To detect this virus efficiently,a SYBR Green I real time fluorescence quantitative PCR(qPCR)method was proposed using a specific primer based on the conserved sequence of the coat protein(CP)gene of BSGFV.The results showed that the optimal concentration of the specific primer BSGFV-qG3F/qG3R of qPCR was 0.2 μmol/L,and the optimal annealing temperature was 59℃in qPCR detection.The qPCR achieved a detection limit of 1.0×102 copies/μL,100-fold more sensitive than PCR.The coefficient of variation for qPCR Ct values was less than 5%.The qPCR method was suitable for detecting BSGFV in major cultivars like Brazilian bananas(AAA)but unsuitable for hybrids containing the B genome.This study lays the foundation for the diagnosis,prevention,and control of BSGFV in major banana cultivars in the field.关键词
香蕉线条病毒/香蕉线条病毒GF/内源性香蕉线条病毒GF/SYBR Green I实时荧光定量PCRKey words
banana streak virus/banana streak virus GF/endogenous banana streak virus/SYBR Green I real time fluorescence quantitative PCR分类
农业科技引用本文复制引用
LU Yongsi,LIU Runpei,MAO Zixian,RAO Xueqin..香蕉线条病毒GF SYBR Green Ⅰ实时荧光定量PCR检测方法的建立[J].仲恺农业工程学院学报,2026,39(1):11-15,5.基金项目
国家现代农业产业技术体系项目(CARS-31). （CARS-31）
