中国中药杂志2025，Vol.50Issue(24)：6861-6867,7.DOI:10.19540/j.cnki.cjcmm.20250901.201
裸花紫珠叶中二萜类成分及其抗炎活性研究
Diterpenoids from leaves of Callicarpa nudiflora and their anti-inflammatory activities
摘要
Abstract
The chemical constituents and anti-inflammatory activities of the leaves of Callicarpa nudiflora were investigated using various chromatographic separation methods.Eight diterpenoids were isolated from the ethyl acetate extract and identified by MS and NMR data analysis as calnudiflopene A(1),(5S,9S,10S,12S,13R)-3,4-seco-12,13-dihydroxy-4(18),8(17),14(15)-labdatrien-3-oic acid(2),3,4-seco-12R,13S-dihydroxy-4(18),8(17),14(15)-labdatrien-3-oic acid(3),nudiflopene B1(4),nudifloid F(5),(5S,9S,10S)-3,4-seco-16-carbonyl-15,16-epoxy-4(18),8(17),11(E),13(14)-labdatetraen-3-oic acid(nudiflopene G1)(6),callicarpaolide(7),and 7α-hydroxysandaracopimaric acid(8).Among them,compound 1 is a new 3,4-seco-labdane diterpenoid,in which a linear monoterpenoid is ester-linked to compound 2.Compound 2 was obtained by alkaline hydrolysis of compound 1,and its absolute configuration was determined by single-crystal X-ray diffraction,thereby establishing the absolute configuration of compound 1.The anti-inflammatory activities of all compounds were evaluated in RAW264.7 cells induced by lipopolysaccharide(LPS).Compounds 1 and 5 exhibited certain activity,with IC50 values of(22.8±0.52)and(31.2±0.85)μmol·L-1,respectively.关键词
裸花紫株/半日花烷型二萜/3,4-裂环/水解反应/抗炎活性Key words
Callicarpa nudiflora/labdane diterpenoids/3,4-seco/hydrolysis reaction/anti-inflammatory activities引用本文复制引用
HU Kang-ping,LU Jin-lei,LIU Yu-juan,SONG Xin-ming,YU Zhang-xin,CHEN Guang-ying..裸花紫珠叶中二萜类成分及其抗炎活性研究[J].中国中药杂志,2025,50(24):6861-6867,7.基金项目
海南省重点研发项目(ZDYF2022SHFZ286) （ZDYF2022SHFZ286）
海南师范大学大学生创新训练项目(202511658007) （202511658007）
