中国烟草科学2025，Vol.46Issue(6)：16-22,7.DOI:10.13496/j.issn.1007-5119.2025.06.003
外源茉莉酸甲酯对烟苗烟碱合成、积累和分配的影响
Effects of Exogenous Methyl Jasmonate on Synthesis,Accumulation and Distribution of Nicotine in Tobacco Seedlings
摘要
Abstract
This study investigated the effects of exogenous methyl jasmonate(MeJA)on nicotine synthesis and distribution in different parts of tobacco seedlings.Using flue-cured tobacco varieties Zhongchuan208 and Zhongyan100 as test materials,a two-factor(variety×concentration)completely randomized design was adopted,with three MeJA concentrations:0.1 mg/L(M1),1 mg/L(M2),10 mg/L(M3),and a control(CK,sprayed with water mixed with solvent).The results showed that MeJA application significantly increased nicotine content and accumulation in all parts of the seedlings,with the most pronounced enhancement observed at 1 mg/L(M2).Compared to CK,Zhongchuan208 exhibited increases of 0.17,0.19,0.35,0.38,and 0.51 percentage points in nicotine content in roots,stems,lower leaves,middle leaves,and upper leaves,respectively,while Zhongyan100 showed increases of 0.13,0.21,0.39,0.61,and 0.71 percentage points in the corresponding parts.Nicotine accumulation in Zhongchuan208 increased by 54%,26%,59%,52%,and 66%in roots,stems,lower leaves,middle leaves,and upper leaves,respectively,while Zhongyan100 displayed increases of 41%,32%,50%,75%,and 61%.MeJA upregulated the expression of key nicotine synthesis enzyme genes,NtPMT and NtQPT.It also significantly enhanced the nicotine synthesis rates per unit root length and per unit root mass of the two tobacco varieties.Under the 1 mg/L(M2)treatment,these rates increased by 60%and 66%in Zhongchuan208,and by 76%and 78%in Zhongyan100,respectively.However,MeJA had no significant effect on nicotine distribution proportion among different parts.In conclusion,1 mg/L MeJA is the optimal concentration for promoting nicotine synthesis,as it significantly increases nicotine accumulation while maintaining a stable distribution patterns.This study can provide a theoretical basis for nicotine regulation in flue-cured tobacco.关键词
茉莉酸甲酯/烟碱/分配比例/合成速率/基因表达Key words
methyl jasmonate/nicotine/distribution proportion/synthesis rate/gene expression分类
农业科技引用本文复制引用
WU Qiangwen,YAO Heshuan,YAN Huifeng,LIU Keqiang,HE Yingyu,XU Yang,LI Shuaibing,WANG Yong,SU Yan,LI Jiuzhou,ZHAO Min..外源茉莉酸甲酯对烟苗烟碱合成、积累和分配的影响[J].中国烟草科学,2025,46(6):16-22,7.基金项目
中国农业科学院科技创新工程(ASTIP-TRIC03) （ASTIP-TRIC03）
黑龙江烟草工业有限责任公司科技项目(KJXM2023-02) （KJXM2023-02）
中国烟草总公司山东省公司科技项目(202309) （202309）
