中国烟草科学2025，Vol.46Issue(6)：31-39,9.DOI:10.13496/j.issn.1007-5119.2025.06.005
翻耕深度与起垄高度对烟株根际土壤微生物群落的影响
Effects of Tilling Depth and Ridging Height on Rhizosphere Soil Microbial Community in Tobacco Field
摘要
Abstract
In order to explore the mechanism of different cultivation methods in reducing the harmful of tobacco root-stem diseases,the effects of different tillage depths(20,40,60 cm)and ridge heights(30,40 cm)on the occurrence of tobacco bacterial wilt(TBW)as well as the structure of the rhizosphere soil microbial community were analyzed in this study,with a view to providing a theoretical basis for improving the soil ecological environment through cultivation measures.The results showed that compared with the control,increasing tillage depth and ridge height could significantly reduce the occurrence of TBW,and improve the alpha diversity and beta diversity indexes of soil microbial communities.The Chao1 index,OTUs number,and Shannon index of soil bacterial communities increased by 25.51%-36.03%,25.62%-36.07%,and 10.74%-15.37%,respectively.Corresponding indices of fungal communities increased by 18.08%-101.12%,18.16%-102.20%,and 48.06%-92.97%,respectively.Deep tillage and ridge height had significant impacts on the composition of soil microbial communities.At the bacterial phylum level,increasing the tillage depth and ridge height led to a significant decrease in the relative abundance of Proteobacteria and a significant increase in the relative abundances of Chloroflexi,Gemmatimonadota and Actinobacteriota.At the fungal phylum level,the relative abundance of Ascomycota significantly decreased and the relative abundances of Mortierellomycota significantly increased in the soil by increasing the tillage depth and ridge height.At the genus level,the relative abundance of bacteria and fungi significantly changed under different tilling depths and ridge heights,showing different trends.The optimal tillage depth and ridge height are both 40 cm.Under this condition,the incidence rate of TBW is the lowest.Both the alpha and beta diversity of the soil microbial communities are significantly increased,and the relative abundances of biocontrol bacteria such as Streptomyces and growth promoting bacteria such as Dongia are also significantly increased,which is beneficial for the growth and development of tobacco and reduces the occurrence of tobacco diseases.关键词
翻耕/起垄/土壤微生物/群落结构Key words
tilling/ridging/soil microorganisms/community structure分类
农业科技引用本文复制引用
QIN Yao,LI Lingfeng,ZHANG Yiqian,XIANG Haibo,LI Yanyan..翻耕深度与起垄高度对烟株根际土壤微生物群落的影响[J].中国烟草科学,2025,46(6):31-39,9.基金项目
中国烟草总公司重大科技项目[110202101045(LS-05)、110202201023(LS-07)] （LS-05）
