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烟草靶斑病菌中真菌病毒多样性分析

LI Ping LIU Tianbo LI Sijun YANG Zhijuan SU Jia'en CHEN Yi ZHONG Jie XIAO Yansong

中国烟草科学2025，Vol.46Issue(6)：40-50,59,12.

中国烟草科学2025，Vol.46Issue(6)：40-50,59,12.DOI:10.13496/j.issn.1007-5119.2025.06.006

烟草靶斑病菌中真菌病毒多样性分析

Mycoviral Diversity of Phytopathogenic Fungus Rhizoctonia solani,the Pathogen of Tobacco Target Spot

LI Ping ¹LIU Tianbo ²LI Sijun ³YANG Zhijuan ⁴SU Jia'en ⁴CHEN Yi ⁵ZHONG Jie ⁶XIAO Yansong³

作者信息

1. Yongzhou Institute of Agricultural Sciences,Yongzhou 425006,Hunan,China||College of Plant Protection,Hunan Agricultural University,Changsha 410128,China
2. Hunan Tobacco Science Research Institute,Changsha 410010,China
3. Chenzhou Branch of Hunan Tobacco Company,Chenzhou 423000,Hunan,China
4. Dali Branch of Yunnan Tobacco Company,Dali 671000,Yunan,China
5. Yunnan Academy of Tobacco Agricultural Sciences,Kunming 650031,China
6. College of Plant Protection,Hunan Agricultural University,Changsha 410128,China
折叠

摘要

Abstract

To understand the diversity of mycovirus in the pathogen causing tobacco target spot,dsRNA screening and high-throughput sequencing were performed on the pathogen.Homology searches and phylogenetic analyses were conducted on the obtained candidate viral sequences to determine the viral species and taxonomic status,the analyses of growth rate and pathogenicity were carried out on the virus-carrying strains.The results showed that among 148 strains of the tobacco target spot,14 strains carried dsRNA,which were identified as 30 distinct mycoviruses via sequencing.They belonged to five viral families:Barnaviridae,Benyviridae,Mitoviridae,Narnaviridae,and Partitiviridae.Comparison of growth rate and pathogenicity revealed that no significant difference was observed in growth rate among the virus-carrying strains,yet their virulence exhibited differentiation.The effects of different viruses and their combinations on the function of host strains remain to be further explored.

关键词

真菌病毒/高通量测序/真菌病毒多样性/立枯丝核菌/dsRNA

Key words

mycovirus/high-throughput sequencing/mycoviral diversity/Rhizoctonia solani/dsRNA

分类

农业科技

引用本文复制引用

LI Ping,LIU Tianbo,LI Sijun,YANG Zhijuan,SU Jia'en,CHEN Yi,ZHONG Jie,XIAO Yansong..烟草靶斑病菌中真菌病毒多样性分析[J].中国烟草科学,2025,46(6):40-50,59,12.

基金项目

湖南省自然科学基金(2023JJ30302) （2023JJ30302）

湖南省烟草公司郴州市公司烟草农业科技创新项目(CZYC2024JS05) （CZYC2024JS05）

中国烟草科学

OA北大核心

ISSN：1007-5119

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