中国烟草科学2025，Vol.46Issue(6)：51-59,9.DOI:10.13496/j.issn.1007-5119.2025.06.007
贝莱斯芽孢杆菌D-3A可湿性粉剂研制及对烟草青枯病的防效
Development of Bacillus velezensis D-3A Wettable Powder and Its Control Efficacy against Tobacco Bacterial Wilt
摘要
Abstract
Bacillus velezensis D-3A,an elite biocontrol strain,was studied for the purpose of optimizing its formulation into a wettable powder(WP)for better application and utilization.A single-factor experimental design was employed to identify the carrier,adjuvant,and their composite ratio that minimally impacted the activity of B.velezensis D-3A.Field trials were conducted to assess the efficacy of the formulated WP against tobacco bacterial wilt caused by Ralstonia solanacearum.The optimal formulation and proportion of the B.velezensis D-3A WP were determined as follows:85%kaolin as the carrier,4.8%Tween 80,7.2%polyethylene glycol,2%sodium carboxymethyl cellulose,and 1%sorbitol.The viable count of the preparation was 1.37×1010 cfu/g,fineness 96%,wetting time 14.49 s,suspension rate 95.48%,drying reduction 2.67%,pH 7.52,and impurity rate 0,all of which complied with national standards.The results of continuous storage experiments demonstrated that after 180 days of storage,the spore survival rate reached 85.85%at 4℃and 79.30%at 25℃.Field trials demonstrated that the B.velezensis D-3A WP achieved disease control efficacies of 50.98%and 53.64%on the 7th and 14th days post-treatment,respectively.The B.velezensis D-3A WP exhibits promising efficacy against tobacco bacterial wilt,indicating its potential for further development and application.关键词
烟草/青枯病/贝莱斯芽孢杆菌/可湿性粉剂/防效Key words
tobacco/bacterial wilt/Bacillus velezensis/wettable powder/control efficacy分类
农业科技引用本文复制引用
SHI Chunlan,XU Weixue,WU Guoxing,YANG Dewei,XIE Ziwei,GU Xiaofei,XIE Yonghui,SHI Dingguo,ZHOU Xianfeng,QI Jun,JIANG Dinghui..贝莱斯芽孢杆菌D-3A可湿性粉剂研制及对烟草青枯病的防效[J].中国烟草科学,2025,46(6):51-59,9.基金项目
昆明市烟草公司科技项目(KMYC20220l) （KMYC20220l）
