中国烟草科学2025，Vol.46Issue(6)：60-69,77,11.DOI:10.13496/j.issn.1007-5119.2025.06.008
湖南湘西州烤烟新根茎部病害的病原鉴定
Identification of Pathogens Causing Novel Stem and Root Diseases on Flue-cured Tobacco in Xiangxi,Hunan
摘要
Abstract
In recent years,newly emerging root and stem diseases have been observed in certain major tobacco-growing areas of Xiangxi Prefecture,Hunan Province,and are showing a spreading trend.Therefore,there is an urgent need for accurate identification of the new diseases and precise characterization of their causal agents.To achieve this,we conducted field observations of typical symptoms,pathogen isolation,morphological characterization,Koch's postulates verification,molecular identification,and phylogenetic analysis.The results showed as follows.The newly occurred Fusarium root rot in Baojing County was caused by Fusarium oxysporum and F.solani,with F.oxysporum as the predominant pathogen.The root and stem disease causing"black spots"on tobacco roots/stems and aboveground growth retardation in Yongshun County was attributed to Rhizoctonia solani Kühn AG-4 HGI anastomosis group;the pathogen was pathogenic to corn,soybean,chickpea,and cotton plants under artificial inoculation conditions.The pathogenic bacterium Ralstonia solanacearum isolated from Baojing,Huayuan,and Longshan Counties was identified as Phylotype Ⅰ,with Sequevar 17(Huayuan)and Sequevar 44(Baojing and Longshan).Notably,Sequevar 44 was the first report in this region.This study provides a scientific basis for the accurate diagnosis and control of the newly emerging root and stem diseases of flue-cured tobacco in Xiangxi Prefecture,as well as for crop selection under the new mode of grain-tobacco integrated planting.关键词
烟草根茎病害/病害诊断/立枯丝核菌菌丝融合群/青枯菌序列变种/镰刀菌Key words
tobacco root and stem disease/disease diagnosis/Rhizoctonia solani anastomosis group/Ralstonia solanacearum sequevar/Fusarium spp.分类
农业科技引用本文复制引用
WANG Jing,CAI Xianjie,GENG Mingyan,CHAO Jin,KONG Fanyu,WANG Xiaoqiang,ZHU Junwu,CHEN Fuliang,CAO Shoutao,TENG Kai..湖南湘西州烤烟新根茎部病害的病原鉴定[J].中国烟草科学,2025,46(6):60-69,77,11.基金项目
上海烟草集团有限责任公司科技项目(2022310000140736) （2022310000140736）
中国农业科学院科技创新工程(ASTIP-04) （ASTIP-04）
