中国畜禽种业2025，Vol.21Issue(12)：21-29,9.
牛奶基因组DNA提取方法的建立及其在牛品种鉴定与个体识别中的应用
Establishment of a milk genomic DNA extraction method and application in bovine breed identification and individual recognition
Liu Jiqiang 1Niu Hongbo 1Hao Xiaodong 1Wang Yanyan 1Dai Wei1
作者信息
- 1. Beijing Compass Agritechnology Co.Ltd.,102200,Beijing
- 折叠
摘要
Abstract
The objective of this study was to obtain high-quality genomic DNA from an appropriate volume of milk and apply it to bovine breed identification and individual recognition.Briefly,6 mL of milk was centrifuged at 4000 r/min and 3～5℃for 15～25 min;the upper fat layer and middle skim layer were discarded,and the bottom pellet was retained.The pellet was then resuspended in sterile PBS buffer(pH value 7.3～7.5)to generate a resuspension,which was again centrifuged at 4000 r/min and 3～5℃for 8～12 min;the supernatant was removed,and the final pellet was collected.Genomic DNA was extracted from these cells using a magnetic-bead protocol compatible with automated platforms,thereby increasing throughput,shortening processing time,and reducing labor costs.Results showed that this method yields single-band,non-degraded,high-quality DNA from only 6 mL of milk,although yields vary among individuals due to differences in somatic cell counts.The extracted DNA meets the requirements for gene-chip genotyping and next-generation sequencing.关键词
牛奶/DNA提取/基因芯片检测/个体识别/品种鉴定Key words
Milk/DNA extraction/Gene chip testing/Individual identification/Variety identification分类
农业科技引用本文复制引用
Liu Jiqiang,Niu Hongbo,Hao Xiaodong,Wang Yanyan,Dai Wei..牛奶基因组DNA提取方法的建立及其在牛品种鉴定与个体识别中的应用[J].中国畜禽种业,2025,21(12):21-29,9.
