中国现代中药2025，Vol.27Issue(12)：2234-2240,7.DOI:10.13313/j.issn.1673-4890.20250325002
天麻花杆中的化合物抗胶质瘤活性评价
Evaluation of Anti-glioma Activity of Compounds from Flowering Branches of Gastrodia elata
摘要
Abstract
Objective:To study the anti-glioma activity of morins and mulberrofurans from the flower branch of Gastrodia elata.Methods:The glioma HS683 cells were used,and the anti-glioma activity of sogastrodinol(1),gastrodinol B(2),gastrodinol C(3),isotetrapterol(4),cyclomorusinol hydroperoxide(5),benzylkuwanon C(6),and benzylmulberrofuran G(7)from the flowering branch of G.elata was investigated by cell proliferation assay(MTS),cell scratch assay,and flow cytometry-based cell cycle analysis methods.Results:Compounds 2-5 and 7 significantly inhibited the proliferation of glioma HS683 cells,with their half inhibitory concentration(IC50)values of 63.54,26.99,12.90,12.17,and 11.73 μmol·L-1,respectively.Moreover,the results of the cell scratch assay showed that the morins 4,5 and mulberrofuran 7 exhibited a substantial inhibitory effect on the migration of HS683 cells.Further cell cycle analysis revealed that compounds 4 and 7 induced a significant M phase arrest in HS683 cells,while compound 5 induced a significant G1 phase arrest in HS683 cells,thereby exerting anti-glioma activity.Conclusion:Compounds 4,5,and 7 showed the most significant anti-glioma activity,and they have the potential to be used as lead molecules.This study provides a scientific theoretical basis for the targeted development of the flowering branch of G.elata resources and anti-glioma active products,as well as drug development.关键词
天麻花杆/小分子化合物/胶质瘤细胞/细胞迁移/细胞周期阻滞Key words
flowering branch of Gastrodia elata/small molecule compound/glioma cell/cell migration/cell cycle arrestt分类
医药卫生引用本文复制引用
ZHANG Zhongxin,QIN Shihui,LIU Qingbo,YANG Liu,JIA Jianke,ZHOU Zeqin,SUN Tianzhu,HU Jiangmiao..天麻花杆中的化合物抗胶质瘤活性评价[J].中国现代中药,2025,27(12):2234-2240,7.基金项目
云南省科技厅科技计划项目(202401AS070075) （202401AS070075）
