中医药信息2026，Vol.43Issue(1)：1-7,7.DOI:10.19656/j.cnki.1002-2406.20260101
好忘方对血管性痴呆大鼠学习记忆障碍的影响及机制研究
Effect and Mechanism of Haowang Formula on Learning and Memory Impairment in Rats with Vascular Dementia
摘要
Abstract
Objective:To investigate the effect and mechanism of Haowang Formula on learning and memory impairment in rats with vascular dementia(VD).Methods:Seventy male SD rats underwent adaptive feeding for one week before the study.Ten rats were randomly assigned to the sham surgery group,in which only the common carotid artery was isolated without ligation.The remaining rats were subjected to sequential ligation of the common carotid artery for the modeling of VD.Rats that died or underwent unsuccessful modeling were excluded.The successfully modeled rats were randomly assigned into the following groups(n=10 per group):model group,donepezil hydrochloride group(0.45 mg/kg),low-dose Haowang Formula group(3.96 g/kg),medium-dose Haowang Formula group(7.92 g/kg),high-dose Haowang Formula group(15.84 g/kg).Each group was given corresponding medication by gavage once daily for four weeks,after which the outcome analyses were conducted.The escape latency and cross-platform times in each group were detected by Morris water maze test.Hematoxylin-eosin(HE)staining was employed to detect the damage of hippocampal neurons.TUNEL staining was used to observe cell apoptosis;The levels of hippocampus inflammatory factors in rats were measured by ELISA.Biochemical kits were used to measure the levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)in the hippocampal tissue.RT-PCR was utilized to analyze Caspase-3,Caspase-9,Bcl-2,and Bax mRNA levels in hippocampal tissue.By Western blot assay,the expression of relevant protein was detected.Results:Compared with the sham surgery group,rats in the model group exhibited increase in escape latency(P<0.01),a decrease in the number of platform crossings(P<0.01).Furthermore,hippocampal neuronal layers in the model group were disorganized and reduced in number(P<0.01).In addition,the contents of IL-1β,IL-6 and TNF-α increased(P<0.01),neuronal apoptosis rate increased(P<0.01),levels of SOD and GSH-Px in the hippocampal tissue decreased(P<0.01),contents of MDA increases,the expression of Caspase-3,Caspase-9,Bax mRNA and protein in hippocampus was increased,the expression of Bcl-2 mRNA and protein in hippocampus was decreased(P<0.01).Compared with the model group,rats in all Haowang Formula dose groups and donepezil hydrochloride shortened the escape latency(P<0.05,P<0.01)and increased the number of platform crossings(P<0.05,P<0.01).According to the HE staining,the number of hippocampal neuron cells increased with normal morphology and well-arranged alignment,the contents of IL-1β,IL-6 and TNF-α decreased(P<0.05,P<0.01),the number of neuronal apoptosis decreased remarkably(P<0.05,P<0.01),the activities of SOD and GSH-Px in the hippocampal tissue increased,the content of MDA decreased(P<0.05,P<0.01).Moreover,it showed significantly reductions in Caspase-3,Caspase-9,Bax mRNA and protein expression(P<0.05,P<0.01),and significant increases in Bcl-2 mRNA and protein expression(P<0.05,P<0.01).Conclusion:Haowang Formula can improve learning and memory impairments in VD rats by inhibiting neuronal apoptosis,neuroinflammation and oxidative stress.关键词
好忘方/血管性痴呆/学习记忆障碍/神经炎症/神经元凋亡/氧化应激Key words
Haowang Formula/Vascular dementia/Learning and memory impairment/Neuroinflammation/Neuronal apoptosis/Oxidative stress引用本文复制引用
ZHANG Yunhui,YANG Menglin,LI Yonghua,WU Dahua,YANG Kun,LIU Xia,CHENG Yan..好忘方对血管性痴呆大鼠学习记忆障碍的影响及机制研究[J].中医药信息,2026,43(1):1-7,7.基金项目
国家自然科学基金项目(81874462) （81874462）
重庆市中医药传承创新团队-三峡库区道地药材保护与利用多学科交叉创新团队(CXTD202303) （CXTD202303）
重庆市级乡村振兴对口帮扶科技项目(2023TIAD-ZXX0010) （2023TIAD-ZXX0010）
重庆三峡医药高等专科学校呼吸和老年疾病中医药防治创新研究中心(2024年) （2024年）
重庆三峡医药高等专科学校中医药防治呼吸和老年病团队项目(TIG202302) （TIG202302）
