中医药信息2026，Vol.43Issue(1)：8-12,5.DOI:10.19656/j.cnki.1002-2406.20260102
生、制木香制备香连散对腹泻小鼠水通道蛋白和Na+-K+-ATP酶的影响
Effects of Xianglian Powder Prepared from Raw and Processed Aucklandiae Radix on Aquaporins and Na+-K+-ATPase in Diarrhea Mice
摘要
Abstract
Objective:To compare the pharmacodynamic differences and mechanisms of action of Xianglian Powder prepared from raw and processed Aucklandiae Radix against diarrhea.Methods:Fifty-six SPF-grade male Kunming mice were randomly divided into a blank group,a model group,a raw-high-dose group(0.771 4 g/mL),a raw-low-dose group(0.385 7 g/mL),a processed-high-dose group(0.771 4 g/mL),a processed-low-dose group(0.385 7 g/mL),and a positive group(loperamide hydrochloride 2 mg/kg).Except for the blank group,mice in the other groups were administered senna leaf decoction(0.1 mL/10 g)by gavage for 7 consecutive days to establish the model.After successful modeling,corresponding drug treatments were administered for 4 consecutive days.Simultaneously,0.5 hours after the last administration,the diarrhea model was reinforced using senna leaf.After modeling,mice were placed in metabolic cages to record the total number of feces,the number of loose stools,and the loose stool grade,and to calculate the loose stool rate,average loose stool grade per mouse,diarrhea rate,and diarrhea index.After fasting for 12 hours with free access to water,blood was collected on the 11th day after the final administration.Serum Na+-K+-ATPase activity and the expression levels of aquaporins AQP3 and AQP4 were detected by enzyme-linked immunosorbent assay(ELISA).Results:The average diarrhea index(0.29±0.3)and average diarrhea rate(47.8%)in the processed-high-dose group were significantly lower compared to the model group(P<0.05).Na+-K+-ATPase activity increased in the processed-high-dose group(17.60±2.50),which was significantly higher than those in the model group(P<0.05).The expression level of aquaporin AQP3 in the serum of mice in the processed-high-dose group was up-regulated(156.45±6.39),significantly higher than in the model group(P<0.01).The expression level of aquaporin AQP4 was up-regulated(715.24±73.44),significantly higher than in the model group(P<0.001).Compared with the processed-high-dose group,the diarrhea index on the third day showed a significant difference in the raw-product groups(P<0.05);serum Na+-K+-ATPase activity in the raw-low-dose group showed a significant decrease(P<0.05);and the expression level of AQP4 in the raw-high-dose group was significantly up-regulated(P<0.05).Conclusion:The antidiarrheal effects of the high-dose processed Xianglian Powder groups are superior to that of the raw Xianglian Powder groups.Its mechanism may involve increasing Na+-K+-ATPase activity levels and up-regulating the expression levels of aquaporins AQP3 and AQP4 to achieve the antidiarrheal effect.关键词
麸煨木香/香连散/腹泻/Na+-K+-ATP酶/水通道蛋白Key words
Bran-processed Aucklandiae Radix/Xianglian Powder/Diarrhea/Na+-K+-ATPase/Aquaporin引用本文复制引用
HUANG Xueting,SONG Deqin,DU Ran,YU Xinyan,ZHANG Xu..生、制木香制备香连散对腹泻小鼠水通道蛋白和Na+-K+-ATP酶的影响[J].中医药信息,2026,43(1):8-12,5.基金项目
辽宁省大学生创新创业项目(S202210162007) （S202210162007）
