中医学报（英文）2025，Vol.5Issue(4)：242-251,10.DOI:10.1055/s-0045-1814107
IGF2BP3作为HNSCC中的关键调控基因的鉴定及其潜在抑制剂的虚拟筛选研究
Targeting IGF2BP3 in HNSCC:Identification and Virtual Screening of Inhibitors
摘要
Abstract
Background Resistance to traditional intense chemotherapy and high invasiveness are characteristics of head and neck squamous cell cancer(HNSCC).Numerous human disorders are linked to N6-methyladenosine(m6A)modification of RNA,and the genetic changes in m6A regulatory genes in HNSCC are not well-understood.There is also a pressing need to find efficient targets and inhibitors for the treatment of HNSCC.This investigation examined the RNA m6A alteration in HNSCC and found putative IGFBP3 inhibitors for potential use. Methods We examined m6A regulator gene expression data from the public Gene Expression Omnibus(GEO)database in both normal tissues and patient HNSCC.For bioinformatics analysis,the R package and additional tools,including the m6A2Target database,Gene Ontology(GO)functional and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses,Gene Expression Profiling Interactive Analysis(GEPIA),and Human Protein Atlas,were used to examine the molecular mechanisms and prognostic impact for regulators that are distinctly expressed.Furthermore,TCMBank molecules were employed for virtual screening to find possible inhibitors. Results Out of the 151 differentially expressed genes(DEGs)found in the chosen datasets,insulin-like growth factor 2 mRNA-binding protein 3(IGF2BP3)was the m6A regulator that was elevated in HNSCC.The GO and KEGG analyses revealed that the target genes of IGF2BP3 were mostly enriched in several pathways and activities linked to cancer.In patients with HNSCC,IGF2BP3 showed an effective predictive effect by GEPIA analysis.Virtual screening yielded four traditional Chinese medicine ingredients as putative IGF2BP3 inhibitors for additional research. Discussion This work learned the role and prognostic effect of IGF2BP3 in HNSCC;meanwhile,the potential inhibitors of IGF2BP3 were identified for further study.关键词
头颈鳞状细胞癌/IGF2BP3/预后/抑制剂/虚拟筛选Key words
head and neck squamous cell carcinoma/IGF2BP3/prognosis/inhibitors/virtual screening引用本文复制引用
Guoyi Yan,Shouhu Li,Yonghua Qi,Ge Liu,Wei Zhao,Zhenjiang Qiu,Mingyuan Wu,Mengyao Luo,Shuyi Yan,Yange Xing,Muhammad Noman Khan..IGF2BP3作为HNSCC中的关键调控基因的鉴定及其潜在抑制剂的虚拟筛选研究[J].中医学报（英文）,2025,5(4):242-251,10.基金项目
National Natural Science Foundation of China(22307109),Natural Science Foundation of Henan Province(222300420510),Key Research Project Plan for Higher Education Institutions in Henan Province(23A350013),and the Tackle Key Problems in Science and Technology Project of Henan Province,China(242102311226,242102310417). （22307109）
