安徽医科大学学报2025，Vol.60Issue(12)：2235-2246,12.DOI:10.19405/j.cnki.issn1000-1492.2025.12.006
MAGED4通过SIRT7激活PI3K/AKT信号通路促进胶质瘤细胞增殖
MAGED4 activates the PI3K/AKT signaling pathway through SIRT7 to promote glioma cell proliferation
摘要
Abstract
Objective To determine the expression of melanoma-associated antigens D4(MAGED4)and SIRT7 in human glioma,and to analyze the potential effects of MAGED4 and SIRT7 on glioma cell proliferation.Methods The MAGED4 and SIRT7 expression levels and their correlation were compared by the China glioma genome atlas(CGGA),human protein atlas(HPA),and UALCAN databases.Survival analysis,ROC curve analysis,and Cox regression analysis were used to predict the outcome of MAGED4 and SIRT7 in glioma patients.Gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)signaling pathway enrichment analysis were used to explore the biological functions of MAGED4 and SIRT7 in glioma.Western blot experiment was used to investigate whether MAGED4 protein exerted its regulatory effects on the activity of the PI3K/AKT signaling pathway via SIRT7.The effect of MAGED4 on cell proliferation in glioma through SIRT7 was explored by CCK-8.Results The analysis results of CGGA,UALCAN,and HPA databases showed that the expression levels of MAGED4 and SIRT7 in glioma tissues were higher than those in normal brain tissue,and the expression were positively correlated.Re-sults of survival,ROC,and Cox analysis showed that high expression of MAGED4 and SIRT7 mRNA were risk fac-tors for poor prognosis in glioma.Results of KEGG enrichment analysis showed that MAGED4 and SIRT7 were as-sociated with the PI3K/AKT signaling in glioma,and Western blot results showed that MAGED4 activated the PI3K/AKT signaling pathway by regulating SIRT7.The CCK-8 results showed that MAGED4 promotes the prolifer-ation of glioma cells through SIRT7.Conclusion MAGED4 and SIRT7 are highly expressed in glioma and associ-ated with poor prognosis,and MAGED4 promotes glioma cell proliferation through activation of the PI3K/AKT sig-naling pathway by SIRT7.关键词
MAGED4/SIRT7/PI3K/AKT/胶质瘤/KEGG富集分析/细胞增殖Key words
MAGED4/SIRT7/PI3K/AKT/glioma/KEGG enrichment analysis/cell proliferation分类
医药卫生引用本文复制引用
叶爱,谢小薰,钟子亮,李枫,谢欢,邹小琼,王国鉴,王紫,罗彬,张庆梅..MAGED4通过SIRT7激活PI3K/AKT信号通路促进胶质瘤细胞增殖[J].安徽医科大学学报,2025,60(12):2235-2246,12.基金项目
国家自然科学基金项目(编号:82260608) （编号:82260608）
广西自然科学基金项目(编号:2022GXNSFAA035639) （编号:2022GXNSFAA035639）
广西研究生教育创新计划项目(编号:YCSW2024251) National Natural Science Foundation of China(No.82260608) （编号:YCSW2024251）
Natural Science Foundation of Guangxi Province(No.2022GXNSFAA035639) （No.2022GXNSFAA035639）
Innovation Project of Guangxi Graduate Education(No.YCSW2024251) （No.YCSW2024251）
