安徽医科大学学报2025，Vol.60Issue(12)：2247-2254,8.DOI:10.19405/j.cnki.issn1000-1492.2025.12.007
DCX过表达胶质瘤细胞中差异基因的生物信息学分析
Bioinformatics analysis of differentially expressed genes in DCX-overexpression glioma cells
摘要
Abstract
Objective To investigate the role mechanism of doublecortin(DCX)in glioma and screen DCX over-expression-related differentially expressed genes,so as to provide new targets for glioma targeted therapy.Methods Using rat glioma C6 cells as the target cells,LV-DCX-EGFP,LV-Cas9-Puro,and LV-Ctrl-EGFP lentiviruses were constructed.DCX overexpressing and control C6 cell lines were established through Cas9 dual-vector system transfection and screening.Transcriptome sequencing was used to obtain the differentially expressed gene profiles.iDEP and DESeq2 were applied to screen differentially expressed genes with the threshold of|Log2FC|＞2 and P＜0.05.STRING and Cytoscape were utilized to construct protein-protein interaction networks and screen key genes.DAVID was employed for gene ontology(GO)enrichment analysis.BUSCA and UniProt were used to predict the subcellular localization of key genes.Receiver operating characteristic(ROC)curves were plotted to verify the di-agnostic value of key genes.Enrichr was adopted to predict the regulatory networks of transcription factors and miR-NAs.RT-qPCR was performed to validate the expression of key genes.Results A total of 45 upregulated and 47 downregulated differentially expressed genes were screened,and 6 upregulated key genes including POU2F3 and 5 downregulated key genes including UBB were identified.GO enrichment analysis showed that upregulated genes were enriched in biological processes such as the positive regulation of lipid biosynthesis,whereas downregulated genes were linked to molecular functions including the regulation of alkaline phosphatase activity.Key genes were mainly distributed in the nucleus and cytoplasm.Except for RPS17,all key genes had an AUC value＞0.7,indi-cating good diagnostic value.RT-qPCR validation showed that the mRNA expression levels of some upregulated genes(POU2F3,LPAR6,SREBF1)significantly increased(P＜0.001),while the mRNA expression levels of some downregulated genes(UBB,ACTB,UBE2I)also significantly increased(P＜0.001),which might be relat-ed to transcriptional and post-transcriptional regulation.Conclusion Differentially expressed genes and their regu-latory networks related to DCX overexpression in glioma are successfully screened,providing a theoretical basis for revealing the role mechanism of DCX in glioma development and laying a foundation for the development of potential therapeutic strategies.关键词
胶质瘤/双皮质素/差异表达基因/生物信息学/富集分析/转录组测序Key words
glioma/doublecortin/differentially expressed genes/bioinformatics/enrichment analysis/RNA se-quencing分类
医药卫生引用本文复制引用
周玲欣,Iqra Nadeem,秦玉侠,熊晔..DCX过表达胶质瘤细胞中差异基因的生物信息学分析[J].安徽医科大学学报,2025,60(12):2247-2254,8.基金项目
国家自然科学基金项目(编号:82273250) National Natural Science Foundation of China(No.82273250) （编号:82273250）
