安徽医科大学学报2025，Vol.60Issue(12)：2255-2263,9.DOI:10.19405/j.cnki.issn1000-1492.2025.12.008
自身抗原诱导的慢性间质肺损伤小鼠模型的建立
The establishment of a mouse model of self-antigen-induced chronic interstitial lung injury
摘要
Abstract
Objective To establish a mouse model of lung injury induced by self-antigens and explore its pathologi-cal mechanisms to provide a reliable animal model for studying the pathogenesis of rheumatoid arthritis-associated interstitial lung disease(RA-ILD).Methods The mice were injected intradermal with antigenemulsion made of complete freund's adjuvant(CFA)and lung tissue protein,and the emulsion prepared with incomplete Freund's adjuvant(IFA)was used to enhance immunity.HE staining was used to observe the histopathological changes in the mice.Masson staining was used to detect pulmonary fibrosis.The mRNA levels of rheumatoid factor(RF),krebs von den lungen-6(KL-6)and surfactant protein D(SP-D)were detected by qPCR.The levels of ACPA,IgG1,IgG2a and IgG3 antibodies were detected by ELISA.The changes of inflammatory cells and α-smooth muscle actin(α-SMA)expression in lung tissue were detected by immunofluorescence staining.The protein expression lev-els of Collagen Ⅰ and α-SMA were detected by Western blot.The changes of immune cells were detected by flow cy-tometry.Results HE staining showed that inflammatory cell infiltration increased and tissue structure changed sig-nificantly in lung tissue after the model was established by self-lung tissue antigen.Masson staining showed in-creased collagen deposition in lung tissue of model mice.qPCR tests revealed elevated mRNA levels of RF,KL-6 and SP-D.ELISA tests revealed elevated levels of ACPA,IgG1 and IgG3 antibodies.Immunofluorescence results showed that monocytes and T cells increased,and α-SMA expression increased in the model group.Western blot results showed increased protein expressions of Collagen Ⅰ and α-SMA.The flow cytometry results showed an in-crease in T cells and monocytes in the lung tissue.Conclusion The mouse model of lung injury induced by self-antigens is successfully established,and T cells and monocytes may be involved in the occurrence and progression of the disease.关键词
自身抗原/动物模型/肺损伤/肺纤维化/T细胞/单核细胞Key words
self-antigens/animal model/lung injury/pulmonary fibrosis/T cells/monocytes分类
医药卫生引用本文复制引用
吴余玲,徐清,王迪,胡姗姗..自身抗原诱导的慢性间质肺损伤小鼠模型的建立[J].安徽医科大学学报,2025,60(12):2255-2263,9.基金项目
安徽省自然科学基金项目(编号:2308085MC78) （编号:2308085MC78）
2020年度抗炎免疫药物教育部重点实验室开放课题(编号:KFJJ-2020-09) Natural Science Foundation of Anhui Province(No.2308085MC78) （编号:KFJJ-2020-09）
Open Project of Key La-boratory of Anti-inflammatory and Immune Medicines,Ministry of Education in 2020(No.KFJJ-2020-09) （No.KFJJ-2020-09）
