安徽医科大学学报2025，Vol.60Issue(12)：2289-2298,10.DOI:10.19405/j.cnki.issn1000-1492.2025.12.012
基于网络药理学、转录组学和实验验证探讨苍耳亭抑制喉鳞状细胞癌细胞增殖的作用机制
Investigating the mechanism of Xanthatin in inhibiting proliferation of laryngeal squamous cell carcinoma cells based on network pharmacology,transcriptomics,and experimental validation
摘要
Abstract
Objective To investigate the potential mechanisms of Xanthatin in inhibiting the proliferation of laryn-geal squamous cell carcinoma(LSCC)cells by integrating network pharmacology and in vitro experiments.Meth-ods The targets of Xanthatin were identified using databases such as PharmMapper,while disease-related targets for LSCC were obtained from databases such as DisGeNET.The overlapping targets between Xanthatin and LSCC were determined by intersecting these datasets.A protein-protein interaction(PPI)network was constructed based on the overlapping targets,and key targets were identified.Gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analyses of the overlapping targets were performed using R software.A"Xantha-tin-target-pathway"network was visualized using Cytoscape 3.8.0 software.The preliminary validation of the afore-mentioned results was performed using molecular docking and transcriptomics.The effects of Xanthatin on the pro-liferation of TU177 cells were assessed using CCK-8 and colony formation assays.Additionally,Western blot analy-sis was employed to measure the expression levels of PI3K,p-PI3K,Akt,and p-Akt proteins.Results A total of 159 overlapping targets between Xanthatin and LSCC were identified,and seven key targets,including AKT1,were screened.GO enrichment analysis yielded 2 455 entries,and KEGG enrichment analysis identified 172 pathways,such as the PI3 K-Akt signaling pathway.Xanthatin exhibited favorable binding activity with the core target proteins of LSCC in molecular docking experiments.The transcriptomics results showed high consistency with the predictions from network pharmacology.CCK-8 and colony formation assays demonstrated that Xanthatin at concentrations of 1,2,and 4 μmol/L significantly inhibited the proliferation of TU177 cells in a dose-dependent manner.The expres-sion levels of p-PI3K and p-Akt proteins decreased with increasing concentrations of Xanthatin.Conclusion Xan-thatin may exert an inhibitory effect on the proliferation of LSCC cells by modulating the PI3K-Akt signaling path-way.关键词
网络药理学/苍耳亭/喉鳞状细胞癌/分子对接/细胞实验/PI3K-Akt信号通路/转录组学/实验验证Key words
network pharmacology/Xanthatin/laryngeal squamous cell carcinoma/molecular docking/cell ex-periments/PI3K-Akt signaling pathway/transcriptomics/experimental validation分类
医药卫生引用本文复制引用
马自创,苏丹,王春,吴娜,王海坤,沈爱宗..基于网络药理学、转录组学和实验验证探讨苍耳亭抑制喉鳞状细胞癌细胞增殖的作用机制[J].安徽医科大学学报,2025,60(12):2289-2298,10.基金项目
安徽省临床医学研究转化专项(编号:202304295107020095) （编号:202304295107020095）
亳州市重点研发计划项目(编号:bzzc2022015) Clinical Medical Research Translational Project of Anhui Province(No.202304295107020095) （编号:bzzc2022015）
Key Research and Development Project of Bozhou(No.bzzc2022015) （No.bzzc2022015）
