生物资源2025，Vol.47Issue(6)：551-555,5.DOI:10.14188/j.ajsh.20251112002
Ⅰ型干扰素受体1基因敲除SD大鼠的遗传表型鉴定
Genetic phenotype identification of type Ⅰ interferon receptor 1-gene knockout SD rats
摘要
Abstract
Type Ⅰ interferon(IFN-I)is a central cytokine in host antiviral immunity,and its signaling pathway is al-so closely associated with the pathogenesis of autoimmune diseases and tumors.Gene knockout animal models are crucial tools for studying the function of this pathway.However,the widely used type Ⅰ interferon receptor 1(IFNAR1)knock-out mouse model has limitations,including species differences and technical constraints,which restrict its translational value.To address this,we successfully generated an Ifnar1 knockout model in Sprague Dawley(SD)rats using CRIS-PR/Cas9 technology.Through genotyping of the offspring,we established a stable strain of Ifnar1-/-homozygous rats.Phenotypic analysis revealed no significant differences in morphology,behavior,or reproductive capability between the knockout and wild-type rats.Furthermore,immunohistochemical staining confirmed the complete absence of IFNAR1 protein expression in multiple tissues of Ifnar1-/-rats,including the liver,spleen,kidney,brain,and reproductive organs.The generation of this Ifnar1 knockout rat model provides a novel and promising preclinical platform for investigating the in vivo functions of the type Ⅰ interferon signaling pathway in a physiological context that more closely recapitulates human biology,and for evaluating the efficacy of related therapeutics.关键词
Ⅰ型干扰素/基因敲除/大鼠模型Key words
type Ⅰ interferon/gene knockout/rat model分类
医药卫生引用本文复制引用
蔡曾,鲜巧阳,包容,张璟怡,苏珊,龙子文,张璋,汤宏斌..Ⅰ型干扰素受体1基因敲除SD大鼠的遗传表型鉴定[J].生物资源,2025,47(6):551-555,5.基金项目
国家重点研发计划(2021YFF0702002),湖北省实验动物研究科技项目(2022DFE022),湖北省自然科学基金(2024AFB103) （2021YFF0702002）
