生物资源2025，Vol.47Issue(6)：565-577,13.DOI:10.14188/j.ajsh.20250729001
连作温郁金和蓬莪术根茎内生细菌群落差异性分析
Differential analysis of endophytic bacterial communities in the rhizomes of continuous cropping of Curcuma wenyujin and C.phaeocaulis
摘要
Abstract
To explore the reasons for the difference in the ability of Curcuma wenyujin and C.phaeocaulis to resist continuous cropping,the rhizomes of C.wenyujin and C.phaeocaulis were collected,high-throughput sequencing was employed to study the community differences of endophytic bacteria in rhizomes of C.wenyujin and C.phaeocaulis.A total of 15 471 operational taxonomic units(OTUs)were obtained from all samples,belonging to 38 phyla,91 classes,260 orders,539 families,and 1 149 genera.Alpha diversity analysis showed that the species richness and deversity of en-dophytic bacteria in the rhizomes of C.phaeocaulis were generally higher than those of C.wenyujin.Continuous crop-ping reduced the richness and diversity of endophytic bacteria in the rhizomes of C.phaeocaulis,while opposite in the rhi-zomes of C.wenyujin.The endophytic bacterial community structure differed between C.wenyujin and C.phaeocaulis,and with the increase of growth time and continuous cropping years,the relative abundance of Proteobacteria increased and Actinobacteria decreased in the rhizomes of C.wenyujin,while opposite in the rhizomes of C.phaeocaulis.Con-tinuous cropping reduced the relative abundance of Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium in C.phaeocaulis,while increasing the relative abundance of Sphingomonas and Streptomyces.However,the relative abun-dance of Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium,and Ralstonia in C.wenyujin increased,while de-creasing the relative abundance of Enterobacter.It can be seen that continuous cropping changes the endophytic bacterial diversity and community structure of C.phaeocaulis and C.wenyujin rhizomes.After continuous cropping,the domi-nant bacterial genera with increased relative abundance in C.phaeocaulis rhizomes may have an antagonistic effect on the obstacles of continuous cropping.关键词
温郁金/蓬莪术/内生细菌/连作障碍/群落结构Key words
Curcuma wenyujin Y.H.Chen et C.Ling/Curcuma phaeocaulis Val./endophytic bacteria/con-tinuous cropping obstacles/community structure分类
农业科技引用本文复制引用
刘海蓉,王德立,金自学..连作温郁金和蓬莪术根茎内生细菌群落差异性分析[J].生物资源,2025,47(6):565-577,13.基金项目
中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-032) （2021-I2M-1-032）
海南省优秀人才团队项目(HNYT20240003) （HNYT20240003）
