草地学报2026，Vol.34Issue(1)：10-22,13.DOI:10.11733/j.issn.1007-0435.2026.01.002
苎麻谷胱甘肽S-转移酶家族全基因组鉴定及其对镉胁迫的响应
Genome-wide Identification of Glutathione S-Transferases(GSTs)Gene Family and Its Responses to Cadmium Stress in Ramie(Boehmeria nivea)
摘要
Abstract
Glutathione S-transferases(GSTs)play an important role in the regulation of plants against stressful environments,but the systematic studies on Ramie were lack.In this paper,a total of 43 BnGSTs gene families were identified by bioinformatic analyses,most of which were predicted to be located in the cytoplasm and belonged to the plant-specific Tau and Phi class.Inter-species covariance analysis revealed that BnGSTs genes represented more orthologous genes in dicots than monocots;intra-species covariance analysis revealed that there were five pairs of paralogous genes among BnGSTs,all of which were derived from the whole genome or fragmentary gene duplication,and all of them were found that Ka/Ks ratios were less than 1,indicating that they were subjected to purifying selection.Cis-acting element prediction revealed that the upstream of these BnGSTs genes was rich in light-responsive,phytohormone-regulated and stress-responsive elements combined with transcriptome expression data,nine tissue-specific candidate genes were screened with significant(P<0.05)changes on expression patterns in roots,stems and leaves under cadmium stress.Two genes,BnGST22 and BnGST12,were identified as having significant up-regulation and high relative expression,which could be used as target genes for further functional verification.This study further enriched the functional gene pool of ramie and provided a theoretical basis for the use of anti-stress resources and variety selection.关键词
苎麻/谷胱甘肽S-转移酶/镉胁迫/生物信息分析Key words
Ramie/Glutathione S-transferases/Cadmium stress/Bioinformatic analysis分类
农业科技引用本文复制引用
颜世伟,顾一舟,赵浩含,梁舒月,高钢,陈平,朱爱国,陈继康..苎麻谷胱甘肽S-转移酶家族全基因组鉴定及其对镉胁迫的响应[J].草地学报,2026,34(1):10-22,13.基金项目
湖南省自然科学基金(2024JJ7622) （2024JJ7622）
国家麻类产业技术体系(CARS-16) （CARS-16）
湖南省湖湘青年英才计划(2022RC1013)资助 （2022RC1013）
