草地学报2026，Vol.34Issue(1)：33-42,10.DOI:10.11733/j.issn.1007-0435.2026.01.004
偏关苜蓿MsMYB44基因的克隆、亚细胞定位及表达分析
Cloning,Subcellular Localization and Expression Analysis of MsMYB44 Gene of Medicago sativa'Pianguan'
摘要
Abstract
The MYB(V-myb Avian myeloblastosis viral Oncogene Homolog)gene family represents one of the most extensive groups of transcription factors in plants,playing a crucial role in various processes including plant growth,development,and stress responses.This study utilized Medicago sativa'Pianguan'to investi-gate the characteristics of the MsMYB44 gene through methodologies such as gene cloning,bioinformatics analysis,subcellular localization,and expression pattern analysis.The findings revealed that the MsMYB44 gene of Medicago sativa'Pianguan'comprised 966 base pairs and encoded a total of 321 amino acids.The MsMYB44 protein exhibited a high degree of homology to the MtMYB44 protein in Medicago truncatula and was localized within the nucleus.The expression of the MsMYB44 gene was observed in various tissues of Medicago sativa'Pianguan'across different developmental stages,indicating tissue-specific expression pat-terns.The MsMYB44 gene was responsive to drought,salt stress,and abscisic acid(ABA)treatment.Under drought conditions,the relative expression level of MsMYB44 initially increased,subsequently decreased,and then increased again,peaking at 12 hours.In response to salt stress,the relative expression level of MsMYB44 gradually increased after 48 hours,reaching its maximum at 72 hours.Following ABA treatment,the relative expression level of MsMYB44 peaked at 12 hours and then progressively declined.These findings would pro-vide a theoretical framework for elucidating the role of the MsMYB44 gene of Medicago sativa in mediating the abiotic stress response.关键词
MsMYB44/'偏关'苜蓿/克隆鉴定/亚细胞定位/表达模式/非生物胁迫Key words
MsMYB44/Medicago sativa'Pianguan'/Cloning identification/Subcellular localization/Expres-sion pattern/Abiotic stress分类
农业科技引用本文复制引用
李迪娜,王凯,陶佳丽,许涛,李青秀,朱慧森,岑慧芳..偏关苜蓿MsMYB44基因的克隆、亚细胞定位及表达分析[J].草地学报,2026,34(1):33-42,10.基金项目
国家自然科学基金项目(32071872) （32071872）
山西省中央引导地方科技发展资金项目(YDZJSX2022B006) （YDZJSX2022B006）
山西省重点研发计划课题(202102140601006-3) （202102140601006-3）
山西省基础研究计划(202303021221101) （202303021221101）
山西省来晋科研奖励(SXBYKY2022118) （SXBYKY2022118）
优秀博士启动项目(2021BQ01,2023BQ03)资助 （2021BQ01,2023BQ03）
