草地学报2026，Vol.34Issue(1)：75-86,12.DOI:10.11733/j.issn.1007-0435.2026.01.008
低温胁迫对黄脊竹蝗卵滞育的消除影响及其转录组分析
The Alimiating Effects of Low-Temperature Stress on Egg Diapause Termination and Transcriptome Analysis in Ceracris kiangsu Tsai
摘要
Abstract
Ceracris kiangsu Tsai is a major pest seriously threatens forestry and grassland ecosystems,with its egg diapause being a critical factor for overwinter survival and population outbreaks.In this study we investi-gated the molecular response mechanisms of egg diapause release in C.kiangsu by applying different low tem-peratures(3℃and 8℃)to the diapausing eggs and combining with transcriptome sequencing.The results showed that the diapause release effect was closely related to the duration and temperature of low-temperature treatment,with a 60-day period being a critical turning point for shortened hatching and increased hatching rates.Hatching rates were significantly higher at 8℃compared to 3℃.Transcriptome analysis revealed that compared to the 25℃control group,3℃and 8℃treatments identified 9730 and 6075 differentially expressed genes(FDR<0.05,|log2FC|>1),respectively.GO and KEGG enrichment analyses revealed that these genes were significantly enriched in metabolic processes,including arginine metabolism,proline metabolism,and insect hormone biosynthesis,and stress responses,including FOXO signaling pathway and MAPK signal-ing pathway.Key genes such as ADAMTSL4,ALDH16A1,and foxo were identified,which regulate meta-bolic processes and developmental progression under low-temperature stress.These findings demonstrate novel insights into the molecular mechanisms of diapause release and highlight the potential for developing new green control targets for managing this pest.关键词
黄脊竹蝗/卵滞育/胚胎发育/转录组Key words
Ceracris kiangsu Tsai/Egg diapause/Embryonic development/Transcriptome分类
生物科学引用本文复制引用
范宇阳,谷汶骏,陈鹏,陈鑫强,张锐,高俨,赵含洋,彭仲谷,文亦芾..低温胁迫对黄脊竹蝗卵滞育的消除影响及其转录组分析[J].草地学报,2026,34(1):75-86,12.基金项目
云南省科技厅重大科技专项(202502AE090016)资助 （202502AE090016）
