南方医科大学学报2026，Vol.46Issue(1)：34-46,13.DOI:10.12122/j.issn.1673-4254.2026.01.04
茯苓多糖通过调节肠道微生物群减轻环磷酰胺引起的肠道屏障和免疫损伤
Poria cocos polysaccharide alleviates cyclophosphamide-induced intestinal barrier dysfunction and inflammation in mice by modulating gut flora
摘要
Abstract
Objective To investigate the protective effects of Poria cocos polysaccharide(PCP)against cyclophosphamide(CTX)-induced intestinal mucosal injury and its impact on gut flora and their metabolites in mice.Methods Adult BALB/C mice were randomized into normal control group,CTX model group,glutamine(positive control)group,and low-,medium-and high-dose PCP treatment groups.In all but the normal control group,the mice were subjected to modeling of CTX-induced intestinal mucosal injury by intraperitoneal CTX injections for 3 days,followed by treatment with gavage of normal saline,glutamine(300 mg/kg),or PCP at 75,150,or 300 mg/kg for 7 consecutive days.The colonic expressions of tight junction proteins(occludin and ZO-1),serum endotoxin,D-lactate,and DAO levels,intestinal permeability,colon injury,and colonic cytokine levels(IL-4,IL-22,IL-17A,and IFN-γ mRNA)were assessed.Gut microbiota,short-chain fatty acids(SCFAs;mainly acetates and propionates)and colonic GPR41 expression were analyzed using 16S rRNA sequencing,GC-MS,and Western blotting,respectively.Fecal microbiota transplantation(FMT)experiment was conducted to validate the role of gut microbes in PCP-mediated repair of intestinal injuries.Results Compared with those in the model group,the mice treated with PCP showed significantly increased colonic occludin and ZO-1 expressions,reduced serum endotoxin,D-lactate and DAO levels,and lowered intestinal permeability with increased colonic expressions of IL-4,IL-22,IL-17A,and IFN-γ mRNA.PCP treatment obviously increased the abundance of Muribaculaceae,decreased Lactobacillus and Bacteroides,increased the contents of acetate and propionate in the colon,and upregulated colonic GPR41 expression.The results of FMT experiment confirmed the crucial role of gut microbes in PCP-mediated repair of CTX-induced intestinal injuries in mice.Conclusion PCP can protect against CTX-induced intestinal mucosal injury in mice possibly by modulating gut flora and SCFAs metabolism to enhance intestinal defense capacity.关键词
茯苓多糖/肠粘膜损伤/肠道菌群/短链脂肪酸/GPR41Key words
Poria cocos polysaccharide/intestinal mucosal injury/gut flora/short-chain fatty acids/GPR41引用本文复制引用
张越,陈卫东,王妍妍,段雨婷,张晨,喻璐喆,刘颖颖,邢丽花,王雷,俞年军,彭代银..茯苓多糖通过调节肠道微生物群减轻环磷酰胺引起的肠道屏障和免疫损伤[J].南方医科大学学报,2026,46(1):34-46,13.基金项目
国家自然科学基金青年项目(82505171,82204748) （82505171,82204748）
国家自然科学基金区域创新发展联合基金(U25A20173) （U25A20173）
安徽省科技创新攻坚计划项目(202423l10050002) （202423l10050002）
安徽省科技特派员示范项目(皖科生秘[2024]407 号) （皖科生秘[2024]407 号）
高水平中医药重点学科建设项目(zyyzdxk-2023095) （zyyzdxk-2023095）
安徽省高校科研计划项目团队项目(2022AH010036)Supported by Natural Science Foundation for the Youth(NSFY)of China(82505171,82204748). （2022AH010036）
