南方医科大学学报2026，Vol.46Issue(1)：47-54,8.DOI:10.12122/j.issn.1673-4254.2026.01.05
石斛合剂通过调控Sirt3介导的线粒体自噬通路缓解大鼠糖尿病心肌病
Shihu Mixture alleviates diabetic cardiomyopathy in rats by Sirt3-mediated upregulation of myocardial mitochondrial mitophagy pathway
摘要
Abstract
Objective To explore the mechanism of Shihu Mixture(SHM)for improving diabetic cardiomyopathy.Methods Thirty male SD rats were randomized into 3 groups(n=10)for type 2 diabetes mellitus modeling by high-fat and-sugar feeding for 12 weeks and intraperitoneal streptozotocin injection,followed by treatment with daily gavage of normal saline(model group),metformin solution,or SHM extract for 4 weeks,with 10 normally fed rats as the normal control group.Fasting blood glucose and cardiac weight index of the rats were monitored,and their TG,TC,LDL-C,HDL-C,and LDH levels were determined;serum and myocardial levels of BNP,CRP,TNF-α and IL-6 were detected with ELISA.Myocardial pathological changes and ultrastructures of myocardial mitochondria and autophagosomes were examined with HE and Masson staining and transmission electron microscopy.Myocardial expressions of Sirt3,FoxO3a,PINK1,Parkin,P62,and LC3 mRNAs and proteins were detected with RT-qPCR,Western blotting,and immunohistochemistry.Results Compared with those in the control group,the rats in the other 3 groups showed significantly increased fasting blood glucose,cardiac weight index,serum TC,TG,LDL-C,LDH,CRP and BNP levels and myocardial levels of TNF-α and IL-6 with lowered HDL-C level,obvious myocardial and mitochondrial pathologies,and dysregulated expression of Sirt3,FoxO3a,p-FoxO3a,PINK1,Parkin,LC3 and P62.Treatment of the rat models with SHM extract significantly reduced fasting blood glucose level and cardiac weight index,lowered the levels of LDH,CRP,BNP,TNF-α,IL-6,TC,TG,and LDL-C,increased HDL-C level,alleviated myocardial and mitochondrial damages,promoted autophagosome formation,and improved dysregulation of mitochondrial autophagy-related gene expression,showing similar effects to metformin.Conclusion SHM alleviates myocardial damage and improves mitochondrial function in rats with diabetic cardiomyopathy by regulating the mitochondrial autophagy pathway through Sirt3.关键词
糖尿病心肌病/石斛合剂/线粒体自噬/Sirt3途径Key words
diabetic cardiomyopathy/Shihu Mixture/mitophagy/Sirt3 pathway引用本文复制引用
林心君,何昱霖,施红,刘佳绣,胡海霞..石斛合剂通过调控Sirt3介导的线粒体自噬通路缓解大鼠糖尿病心肌病[J].南方医科大学学报,2026,46(1):47-54,8.基金项目
国家自然科学基金(81973827) （81973827）
福建省自然科学基金(2023J01332,2019J01332) （2023J01332,2019J01332）
福建中医药大学校管课题(X2022006)Supported by National Natural Science Foundation of China(81973827). （X2022006）
