南方医科大学学报2026，Vol.46Issue(1)：55-65,11.DOI:10.12122/j.issn.1673-4254.2026.01.06
颈椎病颈椎终板软骨的转录组学特征及针刀干预对FGF18/Akt轴关键分子表达的影响
Transcriptomic characteristics of cervical endplate cartilage in cervical spondylosis and effects of acupotomy on the FGF18/Akt axis with cervical spondylosis
摘要
Abstract
Objective To explore the transcriptomic characteristics of the endplate cartilage of the cervical intervertebral disc in cervical spondylosis(CS)and the effects of acupotomy on expressions of fibroblast growth factor 18(FGF18)/protein kinase B(Akt)axis key molecules.Methods Transcriptomic analyses were performed using bioinformatics methods based on the GEO database.In the animal experiment,24 New Zealand rabbits were randomized equally into control,CS model,and acupotomy groups.In the latter two groups,CS models were established followed 7 days later by acupotomy intervention(once a week for 3 weeks)or no particular treatment.Histopathological changes and cell apoptosis in the intervertebral discs were examined with HE staining and TUNEL assay,and the mRNA expressions of FGF18,FGFR3,and Akt in the intervertebral discs were detected using RT-qPCR;the localization and expressions of FGF18,p-Akt,and Akt proteins were examined with immunohistochemistry.Results The endplate cartilage in CS exhibited numerous differentially expressed genes enriched in the PI3K-Akt signaling,calcium signaling,and Rap1 signaling pathways.CS rabbits showed obvious changes in the cervical spine curvature and joint degenerations,changes in cervical intervertebral disc texture,thinning of the annulus fibrosus,shrinkage or even absence of the nucleus pulposus,and increased apoptotic cells in the endplate cartilage,which were all obviously alleviated after acupotomy.No significant differences were found in the mRNA expressions of FGF18,FGFR3,and Akt in the cervical intervertebral discs among the 3 groups.Acupotomy significantly increased FGF18 and p-Akt protein expressions and reduced the Akt/p-Akt ratio in the cervical endplate cartilage of CS rabbits.Conclusion The cervical endplate cartilage of CS show numerous differentially expressed genes.Acupotomy may delay degenerative changes of the intervertebral discs and improves CS by activating the FGF18/Akt axis to reduce apoptosis of endplate cartilage cells.关键词
颈椎病/针刀/转录组特征/终板软骨/FGF18/Akt轴Key words
cervical spondylosis/acupotomy/transcriptome characteristics/intervertebral disc/FGF18/Akt axis引用本文复制引用
刘福水,王小乐,KHALIUNAA Tumurbaatar,曹奇光,杨煜乾,任长安,朱金超,赵小兰,曹锂,邓彪..颈椎病颈椎终板软骨的转录组学特征及针刀干预对FGF18/Akt轴关键分子表达的影响[J].南方医科大学学报,2026,46(1):55-65,11.基金项目
国家重点研发计划(2023YFC3502701) （2023YFC3502701）
国家自然科学基金(82360940) （82360940）
江西省教育厅科技项目(GJJ2200990) （GJJ2200990）
江西省中医药管理局项目(2023B1191)Supported by National Key Research and Development Program of China(2023YFC3502701) （2023B1191）
National Natural Science Foundation of China(82360940). （82360940）
