福建师范大学学报（自然科学版）2026，Vol.42Issue(1)：20-28,9.DOI:10.12046/j.issn.1000-5277.2025010019
基于钒刺激的假单胞菌处理含钒氨氮废水研究
Purification of Vanadium-Containing Ammonia Nitrogen Wastewater by Pseudomonas sp.under Vanadium Stimulation
摘要
Abstract
To promote green and sustainable development of the vanadium industry,this study employed Pseudomonas sp.to treat vanadium-containing high-concentration ammonia-nitrogen wastewater,aiming to address severe environmental pollution from post-vanadium-precipitation am-monia-nitrogen wastewater,as well as reduced vanadium leaching efficiency and pipeline blockage caused by wastewater reuse in leaching processes.Results showed that when ρ(vanadium)≤100 mg·L-1,it promoted Pseudomonas sp.growth,increased transmembrane potential difference and cell membrane permeability,enhanced negatively charged functional groups(e.g.,C=O,N-H)in extracellular polymeric substances(EPS),and improved bacterial substance transport and NH+4 adsorption capacities.At ρ(vanadium)=100 mg·L-1,the ammonia-nitrogen removal rate increased by 6.64%compared to the vanadium-free control.Vanadium entered bacterial cells through extracellular passive adsorption and intracellular active accumulation,acting as a stimulant to the enzyme system,significantly enhancing nitrification-related enzyme activities:nitrate reduc-tase specific activity increased by 16.25%and ammonia monooxygenase by 123.11%,thereby strengthening nitrification.This study reveals that vanadium synergistically stimulates Pseudomonas sp.enzyme system through"adsorption-accumulation-enzyme activity enhancement-substrate trans-port reinforcement",providing a potential approach for advanced purification of high-concentration ammonia-nitrogen wastewater.关键词
含钒页岩/含钒氨氮废水/假单胞菌/生物脱氮/氨单加氧酶/硝化反应Key words
vanadium-bearing shale/vanadium-containing ammonia nitrogen wastewater/Pseudomonas/biological denitrification/ammonia monooxygenase/nitrification分类
化学化工引用本文复制引用
佘依洹,张一敏,郑秋实,薛楠楠,蔡震雷..基于钒刺激的假单胞菌处理含钒氨氮废水研究[J].福建师范大学学报（自然科学版）,2026,42(1):20-28,9.基金项目
国家重点研发计划项目(2023YFC3903902) （2023YFC3903902）
湖北省科技创新人才及服务专项(2022EJD002) （2022EJD002）
