分析测试学报2026，Vol.45Issue(1)：1-11,11.DOI:10.12452/j.fxcsxb.250517365
基于超表面等离子共振生物传感器的亲和力评价平台开发
Development of a Metasurface Plasmon Resonance Biosensor Platform for Affinity Evaluation
摘要
Abstract
In this study,a new affinity detection and evaluation method is constructed based on the metasurface plasmon resonance(MetaSPR)biosensor,which utilizes a highly sensitive MetaSPR chip and simultaneously constructs a high-precision microfluidic flow-through and high-throughput mi-crotiter plate pipeless affinity detection platform,which makes up for the shortcomings of the existing affinity detection methods.In the study,MetaSPR chips with nanopore array structure were prepared and characterized by scanning electron microscope and enzyme marker for their physical structure and optical properties.The SPR chip was tested by using glycerol solutions with different refractive index-es,and the results showed that the chip was extremely sensitive to changes in refractive index,and a good correlation was presented between the response signal and the change in refractive index(r2=0.995).The kinetic parameters such as binding rate constant(Ka),dissociation rate constant(Kd)and dissociation equilibrium constant(KD)were successfully obtained using the WeSPR series of de-tector for affinity detection of a variety of molecular pairs without the need for complex optical compo-nents and were in agreement with the previously reported data.The results show that both assay plat-forms constructed in this study are capable of monitoring the intermolecular binding and dissociation processes in a highly sensitive,real-time and label-free manner,with excellent accuracy,stability and universality,providing an efficient,sensitive,portable and low-cost technological means for the study of biomolecular interactions,which is of broad application prospects in the fields of life sci-ences,drug discovery and screening.关键词
超表面等离子共振/生物芯片/亲和力/分子相互作用/药物筛选Key words
metasurface plasmon resonance/biochip/affinity/molecular interactions/drug screening分类
化学化工引用本文复制引用
李裕鸿,周翰霖,刘钢,黄丽萍..基于超表面等离子共振生物传感器的亲和力评价平台开发[J].分析测试学报,2026,45(1):1-11,11.基金项目
国家自然科学基金(32471472) （32471472）
广州国家实验室专项项目(GZNL2023A03006) （GZNL2023A03006）
