分析化学2025，Vol.53Issue(12)：2009-2019,11.DOI:10.19756/j.issn.0253-3820.251257
活性甾体激素的色谱-质谱分析方法研究进展
Advances in Chromatography-Mass Spectrometry Analytical Methods for Bioactive Steroid Hormones
摘要
Abstract
Bioactive steroid hormones are key to endocrine balance and metabolic regulation,making their accurate quantification vital for clinical diagnosis and treatment monitoring.However,it is challenging for accurate quantitative analysis of steroid hormones due to their structural similarity and complex sample matrices.Traditional immunoassays are simple but susceptible to matrix interference and cross-reactivity,causing false positives.Chromatography-mass spectrometry(MS)offers high separation efficiency and a broad linear range,enhancing accuracy and reliability.Sample pre-treatments like liquid-liquid and solid-phase extraction help to enrich and purify analytes and reduce matrix effects.Derivatization and isotopic internal standards can improve sensitivity and accuracy.This review summarized current chromatography MS-based methods for quantifying bioactive steroids,focusing on innovative applications,current challenges,and the discussion of sample preparation and derivatization strategies.The development trends of chromatography-MS-based methods in quantitative analysis of steroid hormones were prospected.关键词
甾体激素/免疫分析法/色谱-质谱联用/萃取/衍生化/评述Key words
Steroid hormones/Immunoassays/Chromatography-mass spectrometry/Extraction/Derivatization/Review引用本文复制引用
贾欣,蒋尚颖,王若馨,闫存玉..活性甾体激素的色谱-质谱分析方法研究进展[J].分析化学,2025,53(12):2009-2019,11.基金项目
浙江省重点人才工程领军项目(No.ZX2025000014)资助. Supported by the Leading Project of Zhejiang Province's Key Talent Program(No.ZX2025000014). （No.ZX2025000014）
