分析化学2025，Vol.53Issue(12)：2043-2049,7.DOI:10.19756/j.issn.0253-3820.251290
基于环介导等温扩增和无酶核酸分子线路的百日咳一步法检测平台
A One-Step Detection Platform for Pertussis Based on Loop-Mediated Isothermal Amplification and Enzyme-Free Nucleic Acid Circuits
摘要
Abstract
Respiratory infections are one of the most common types of diseases,among which pertussis is an acute respiratory infection caused by Bordetella pertussis(BP),which is highly contagious and has a long course.Infants and young children,due to their weak immunity,may develop serious complications and even may be life-threatening when infected with pertussis.In this study,a one-step detection platform that integrated loop-mediated isothermal amplification(LAMP)with enzyme-free nucleic acid circuit(catalytic hairpin assembly,CHA),termed as LAMP-CHA,was developed.The target gene was exponentially amplified through LAMP,and the amplicons were subsequently recognized by CHA to generate fluorescence signals.The LAMP-CHA platform enabled detection of as low as 1 copy/μL of the conserved gene of BP,exhibiting high sensitivity and specificity.Application to clinical samples further confirmed its reliability and practicality,underscoring its potential as an efficient and accurate tool for clinical diagnosis.关键词
百日咳/环介导等温扩增/催化发卡型自组装/荧光检测Key words
Pertussis/Loop-mediated isothermal amplification/Catalytic hairpin assembly/Fluorescence detection引用本文复制引用
赵欣,于淳旭,吕佰阳,唐艺丹,刘晓亮,翟雨欣,李艳茹,孟繁峥,李冰凌..基于环介导等温扩增和无酶核酸分子线路的百日咳一步法检测平台[J].分析化学,2025,53(12):2043-2049,7.基金项目
吉林省国家重点实验室重大研究计划项目(Nos.SKL202402017,SKL202302030)、吉林省自然科学基金项目(No.20240101005JJ)、国家自然科学基金项目(Nos.22525405,22474135,22504138)和吴阶平医学基金会科研专项资助基金项目(No.320.6750.2024-03-14)资助. Supported by the Major Project of Jilin Province State Key Laboratory(Nos.SKL202402017,SKL202302030),the Natural Science Foundation of Jilin Province(No.20240101005JJ),the National Natural Science Foundation of China(Nos.22525405,22474135,22504138)and the Clinical Research Special Fund of WuJiePing Medical Foundation(No.320.6750.2024-03-14). （Nos.SKL202402017,SKL202302030）
