分析化学2025，Vol.53Issue(12)：2114-2124,11.DOI:10.19756/j.issn.0253-3820.251279
磁分离双色余辉发光适配体传感器同时检测谷物中的黄曲霉毒素B1和赭曲霉毒素A
Simultaneous Detection of Aflatoxin B1 and Ochratoxin A in Grains Using A Magnetic Separation Dual Color Afterglow Luminescence Aptasensor
摘要
Abstract
Food security is of great importance to national economic development and public health.In this work,a magnetic separation dual color persistent luminescent aptasensor(Apt-PLNPs@cDNA-Fe3O4)was developed to meet the requirements of highly sensitive detection of the co-contamination of aflatoxin B1(AFB1)and ochratoxin A(OTA)in grains.The sensor was constructed by integrating adapter functionalized afterglow luminescent nanoparticles(Apt-PLNPs)with complementary chain modified Fe3O4 magnetic particles(cDNA-Fe3O4).The target molecules AFB1 and OTA were competitively bound to their respective aptamers(Apt1 and Apt2)in the sensor,forming AFB1-Apt1-Zn2GeO4∶Mn2+(ZGM)and OTA-Apt2-Zn1.25Ga1.5Ge0.25O4∶Cr3+,Yb3+,Er3+(ZGGOC)complexes,which promoted the dissociation of cDNA1-Fe3O4 and cDNA2-Fe3O4.This method enabled the persistent luminescent nanoparticle ZGM to release corresponding afterglow luminescence signals at a wavelength of 535 nm and ZGGOC at a wavelength of 700 nm,respectively,thereby achieving rapid,background free,and highly specific quantitative detection of AFB1 and OTA mycotoxin.The experimental results showed that the afterglow luminescence intensity of ZGM and ZGGOC had a good linear relationship with concentrations of AFB1 and OTA in the range of 0.001‒50 ng/mL,with detection limits of 3.4×10-4 and 3.7×10-4 ng/mL,respectively.The prepared Apt-PLNPs@cDNA-Fe3O4 sensor was applied to detection of AFB1 and OTA contents in grain samples,with recoveries from 93.5%to 101.2%.This study provided an innovative and effective detection strategy for early warning of mycotoxin contamination in grains.关键词
适配体传感器/长余辉纳米粒子/磁性分离/赭曲霉毒素A/黄曲霉毒素B1Key words
Aptasensor/Long afterglow nanoparticles/Magnetic separation/Ochratoxin A/Aflatoxin B1引用本文复制引用
黄紫薇,王迎辉,王芳焕,孟哲..磁分离双色余辉发光适配体传感器同时检测谷物中的黄曲霉毒素B1和赭曲霉毒素A[J].分析化学,2025,53(12):2114-2124,11.基金项目
国家自然科学基金项目(No.21966024)和宁夏大学生创新创业训练项目(No.S202510749118)资助. Supported by the National Natural Science Foundation of China(No.21966024),the College Students' Innovation and Entrepreneurship Training Program of Ningxia,China(No.S202510749118). （No.21966024）
