国际老年医学杂志2026，Vol.47Issue(1)：1-7,7.DOI:10.3969/j.issn.1674-7593.2026.01.001
基于秀丽隐杆线虫模型的林下山参抗衰老作用研究
Study on the anti-aging effects of mountain-cultivated ginseng based on the Caenorhabditis elegans model
摘要
Abstract
Objective To evaluate the anti-aging effects of mountain-cultivated ginseng using the Caenorhabditis elegans model and preliminarily investigate its mechanisms through network pharmacology analysis and RT-qPCR experiments.Methods The study employed Caenorhabditis elegans as an aging model organism.The impact of the water extract of mountain-cultivated ginseng on the thermotolerance of N2 wild-type Caenorhabditis elegans was observed using a heat stress challenge assay.Aging-related physiologi-cal indicators were measured,including lifespan,body length,locomotor ability,superoxide dismutase(SOD)and malondialdehyde(MDA)levels,lipofuscin accumulation,and reactive oxygen species(ROS)levels.Gene Ontology functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were performed based on network pharmacology.The"moun-tain-cultivated ginseng-chemical components-intersection targets-aging-signaling pathways"network was construct,followed by prelimi-nary validation of potential signaling pathways via uantitative real-time polymerase chain reaction(RT-qPCR).Results The water ex-tract of mountain-cultivated Ginseng significantly extended Caenorhabditis elegans lifespan,increased survival rates under heat stress,enhanced body length and locomotor activity,elevated SOD levels,and reduced accumulations of MDA,lipofuscin,and ROS,and the differences were statistically significant(P<0.05).Network pharmacological analysis indicates that biological processes exhibit a sig-nificant enrichment of targets;mountain-cultivated ginseng may exert its anti-aging pharmacological effects by modulating multiple sig-naling pathways,including PI3K-AKT,Ras,and HIF-1 signaling pathways.RT-qPCR results confirmed mountain-cultivated ginseng significantly downregulated the mRNA expression of age-1,akt-1,and daf-2,while markedly upregulating daf-16 mRNA expression levels(genes associated with the PI3K-AKT signaling pathway in Caenorhabditis elegans).Conclusion Mountain-cultivated ginseng effectively delays aging in Caenorhabditis elegans,primarily through suppression of the PI3K-AKT signaling pathway.关键词
林下山参/抗衰老/秀丽隐杆线虫/网络药理学Key words
Mountain-cultivated ginseng/Anti-aging/Caenorhabditis elegans/Network pharmacology引用本文复制引用
刘玉伟,张鎏荣,刘麟轩,王嘉,崔宇轩,高鹏,王翠竹,刘金平..基于秀丽隐杆线虫模型的林下山参抗衰老作用研究[J].国际老年医学杂志,2026,47(1):1-7,7.基金项目
吉林省科技发展计划科技创新基地(平台)建设—吉林省科技创新中心项目(YDZJ202402035CXJD) （平台）
