国际老年医学杂志2026，Vol.47Issue(1)：8-14,7.DOI:10.3969/j.issn.1674-7593.2026.01.002
没食子酸改性氧化石墨烯光热治疗微针的制备与评价
Preparation and evaluation of gallic acid-modified graphene oxide microneedles for photothermal therapy
摘要
Abstract
Objective To prepare a photothermal therapeutic microneedle for the treatment of age-related chloasma that in-hibits melanin secretion,and to evaluate its performance and efficacy.Methods GAGO nanocomposites were prepared by modifying the graphene oxide(GO)structure with gallic acid(GA);the synthesis of GAGO was verified using UV-Vis spectrophotometry and Fourier transform infrared spectroscopy;the stability of GO and GAGO in aqueous solution,PBS and RPMI 1640 medium was detected using a Malvern particle sizer;three matrix materials,chitosan,poly-vinyl pyrrolidone(PVP),and polyvinyl alcohol,were screened and the micromolding perfusion method was determined.Their topography was characterized using light microscopy and scanning elec-tron microscopy;mechanical properties of microneedles were tested using an Instronnstron compression-tension tester;skin insertion properties of microneedles were tested using SD rat skin;photothermal conversion properties of microneedles were assessed by an 808 nm near-infrared(NIR)laser;and the in vitro inhibitory effect of GAGO photothermal therapy was verified using B16 melanoma cells.Results GA was successfully loaded on GO and had good stability and physicochemical properties.GAGO-PVP microneedles were successfully constructed,with beautiful appearance,easy pouring and demoulding,intact needle tips without bubbles,and through mechanical properties testing,the loss of efficacy reached 3.66 N/needle,which can penetrate the skin without causing inflam-mation,and has good photothermal conversion properties.Compared with the control group,GAGO could significantly increase the ac-tivity of B16 melanoma cells,decrease tyrosinase activity and inhibit melanin secretion during NIR challenge,and the differences were statistically significant(P<0.001).Conclusion GAGO NIR treatment significantly inhibited B16 melanoma cell viability and mela-nin secretion,and GAGO-PVP microneedles have the potential to be applied in melasma treatment.关键词
氧化石墨烯/没食子酸/黄褐斑/微针/光热疗法Key words
Graphene oxide/Gallic acid/Melasma/Microneedle/Photothermal therapy引用本文复制引用
冯怡帆,晋唯,黎迎,董政起..没食子酸改性氧化石墨烯光热治疗微针的制备与评价[J].国际老年医学杂志,2026,47(1):8-14,7.基金项目
中央本级重大增减支项目"名贵中药资源可持续利用能力建设项目"(2060302) （2060302）
国家自然科学基金项目(82304733) （82304733）
中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-018) （2021-I2M-1-018）
吉林省科技发展计划项目(20230401102YY) （20230401102YY）
